TY - JOUR
T1 - Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device
AU - Urbaczek, Ana Carolina
AU - Leão, Paulo Augusto Gomes Carneiro
AU - De Souza, Fayene Zeferino Ribeiro
AU - Afonso, Ana
AU - Vieira Alberice, Juliana
AU - Cappelini, Luciana Teresa Dias
AU - Carlos, Iracilda Zeppone
AU - Carrilho, Emanuel
PY - 2017/12/1
Y1 - 2017/12/1
N2 - This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (i.e., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our "vein-on-a-chip" mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.
AB - This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (i.e., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our "vein-on-a-chip" mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.
KW - SCANNING-ELECTRON-MICROSCOPY
KW - MESENCHYMAL STEM-CELLS
KW - ORGANS-ON-CHIPS
KW - SURFACE MODIFICATION
KW - SHEAR-STRESS
KW - ELECTROPHORESIS MICROCHIPS
KW - EXTRACELLULAR-MATRIX
KW - ARTERIAL DIAMETER
KW - POLYMER SURFACES
KW - LASER PRINT
UR - http://www.scopus.com/inward/record.url?scp=85028816322&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-11043-0
DO - 10.1038/s41598-017-11043-0
M3 - Article
C2 - 28874818
AN - SCOPUS:85028816322
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 10466
ER -