EMC is required for biogenesis of Xport-A, an essential chaperone of Rhodopsin-1 and the TRP channel

Catarina J. Gaspar, Lígia C. Vieira, Cristiana C. Santos, John C. Christianson, David Jakubec, Kvido Strisovsky, Colin Adrain, Pedro M. Domingos

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The ER membrane protein complex (EMC) is required for the biogenesis of a subset of tail anchored (TA) and polytopic membrane proteins, including Rhodopsin-1 (Rh1) and the TRP channel. To understand the physiological implications of EMC-dependent membrane protein biogenesis, we perform a bioinformatic identification of Drosophila TA proteins. From 254 predicted TA proteins, screening in larval eye discs identified two proteins that require EMC for their biogenesis: fan and Xport-A. Fan is required for male fertility in Drosophila and we show that EMC is also required for this process. Xport-A is essential for the biogenesis of both Rh1 and TRP, raising the possibility that disruption of Rh1 and TRP biogenesis in EMC mutants is secondary to the Xport-A defect. We show that EMC is required for Xport-A TMD membrane insertion and that EMC-independent Xport-A mutants rescue Rh1 and TRP biogenesis in EMC mutants. Finally, our work also reveals a role for Xport-A in a glycosylation-dependent triage mechanism during Rh1 biogenesis in the endoplasmic reticulum.

Original languageEnglish
Article numbere53210
JournalEmbo Reports
Volume23
Issue number1
DOIs
Publication statusPublished - 5 Jan 2022

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