Aedes aegypti on Madeira Island (Portugal): genetic variation of a recently introduced dengue vector

Gonçalo Seixas, Patrícia Salgueiro, Ana Silva, Melina Campos, Carine Spenassatto , Matias Reyes_Lugo, Maria Teresa Lourenço Marques Novo, Paulo Ribolla, João Pedro Soares da Silva Pinto, Carla Alexandra Gama Carrilho da Costa Sousa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The increasing population of Aedes aegypti mosquitoes on Madeira Island (Portugal) resulted in the first autochthonous dengue outbreak, which occurred in October 2012. Our study establishes the first genetic evaluation based on the mitochondrial DNA (mtDNA) genes [cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 4 (ND4)] and knockdown resistance ( kdr ) mutations exploring the colonisation history and the genetic diversity of this insular vector population. We included mosquito populations from Brazil and Venezuela in the analysis as putative geographic sources. The Ae. aegypti population from Madeira showed extremely low mtDNA genetic variability, with a single haplotype for COI and ND4. We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C). These results are consistent with a unique recent founder event that occurred on the island of Ae. aegypti mosquitoes that carry kdr mutations associated with insecticide resistance. Finally, we also report the presence of the F1534C kdr mutation in the Brazil and Venezuela populations. To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes. Given the present risk of Ae. aegypti re-invading continental Europe from Madeira and the recent dengue outbreaks on the island, this information is important to plan surveillance and control measures.
Original languageUnknown
Pages (from-to)3-10
JournalMemórias Do Instituto Oswaldo Cruz
Volume108 Suppl 1
Issue numberNA
DOIs
Publication statusPublished - 1 Jan 2013

Keywords

  • Aedes aegypti
  • dengue
  • knockdown resistance
  • mtDNA

Cite this

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title = "Aedes aegypti on Madeira Island (Portugal): genetic variation of a recently introduced dengue vector",
abstract = "The increasing population of Aedes aegypti mosquitoes on Madeira Island (Portugal) resulted in the first autochthonous dengue outbreak, which occurred in October 2012. Our study establishes the first genetic evaluation based on the mitochondrial DNA (mtDNA) genes [cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 4 (ND4)] and knockdown resistance ( kdr ) mutations exploring the colonisation history and the genetic diversity of this insular vector population. We included mosquito populations from Brazil and Venezuela in the analysis as putative geographic sources. The Ae. aegypti population from Madeira showed extremely low mtDNA genetic variability, with a single haplotype for COI and ND4. We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C). These results are consistent with a unique recent founder event that occurred on the island of Ae. aegypti mosquitoes that carry kdr mutations associated with insecticide resistance. Finally, we also report the presence of the F1534C kdr mutation in the Brazil and Venezuela populations. To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes. Given the present risk of Ae. aegypti re-invading continental Europe from Madeira and the recent dengue outbreaks on the island, this information is important to plan surveillance and control measures.",
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Aedes aegypti on Madeira Island (Portugal): genetic variation of a recently introduced dengue vector. / Seixas, Gonçalo; Salgueiro, Patrícia; Silva, Ana ; Campos, Melina ; Spenassatto , Carine ; Reyes_Lugo, Matias ; Novo, Maria Teresa Lourenço Marques; Ribolla, Paulo ; Pinto, João Pedro Soares da Silva; Sousa, Carla Alexandra Gama Carrilho da Costa.

In: Memórias Do Instituto Oswaldo Cruz, Vol. 108 Suppl 1, No. NA, 01.01.2013, p. 3-10.

Research output: Contribution to journalArticle

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T1 - Aedes aegypti on Madeira Island (Portugal): genetic variation of a recently introduced dengue vector

AU - Seixas, Gonçalo

AU - Salgueiro, Patrícia

AU - Silva, Ana

AU - Campos, Melina

AU - Spenassatto , Carine

AU - Reyes_Lugo, Matias

AU - Novo, Maria Teresa Lourenço Marques

AU - Ribolla, Paulo

AU - Pinto, João Pedro Soares da Silva

AU - Sousa, Carla Alexandra Gama Carrilho da Costa

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Y1 - 2013/1/1

N2 - The increasing population of Aedes aegypti mosquitoes on Madeira Island (Portugal) resulted in the first autochthonous dengue outbreak, which occurred in October 2012. Our study establishes the first genetic evaluation based on the mitochondrial DNA (mtDNA) genes [cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 4 (ND4)] and knockdown resistance ( kdr ) mutations exploring the colonisation history and the genetic diversity of this insular vector population. We included mosquito populations from Brazil and Venezuela in the analysis as putative geographic sources. The Ae. aegypti population from Madeira showed extremely low mtDNA genetic variability, with a single haplotype for COI and ND4. We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C). These results are consistent with a unique recent founder event that occurred on the island of Ae. aegypti mosquitoes that carry kdr mutations associated with insecticide resistance. Finally, we also report the presence of the F1534C kdr mutation in the Brazil and Venezuela populations. To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes. Given the present risk of Ae. aegypti re-invading continental Europe from Madeira and the recent dengue outbreaks on the island, this information is important to plan surveillance and control measures.

AB - The increasing population of Aedes aegypti mosquitoes on Madeira Island (Portugal) resulted in the first autochthonous dengue outbreak, which occurred in October 2012. Our study establishes the first genetic evaluation based on the mitochondrial DNA (mtDNA) genes [cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 4 (ND4)] and knockdown resistance ( kdr ) mutations exploring the colonisation history and the genetic diversity of this insular vector population. We included mosquito populations from Brazil and Venezuela in the analysis as putative geographic sources. The Ae. aegypti population from Madeira showed extremely low mtDNA genetic variability, with a single haplotype for COI and ND4. We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C). These results are consistent with a unique recent founder event that occurred on the island of Ae. aegypti mosquitoes that carry kdr mutations associated with insecticide resistance. Finally, we also report the presence of the F1534C kdr mutation in the Brazil and Venezuela populations. To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes. Given the present risk of Ae. aegypti re-invading continental Europe from Madeira and the recent dengue outbreaks on the island, this information is important to plan surveillance and control measures.

KW - Aedes aegypti

KW - dengue

KW - knockdown resistance

KW - mtDNA

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DO - 10.1590/0074-0276130386

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EP - 10

JO - Memórias Do Instituto Oswaldo Cruz

JF - Memórias Do Instituto Oswaldo Cruz

SN - 1678-8060

IS - NA

ER -