Elemental mapping of biofortified wheat grains using micro X-ray fluorescence

I. Ramos, I. M. Pataco, M. P. Mourinho, F. Lidon, F. Reboredo, M. F. Pessoa, M. L. Carvalho, J. P. Santos, M. Guerra

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


Micro X-ray fluorescence has been used to obtain elemental maps of biofortified wheat grains. Two varieties of wheat were used in the study, Triticum aestivum L. and Triticum durum desf. Two treatments, with different nutrient concentration, were applied to the plants during the whole plant growth cycle. From the obtained elemental maps it was possible to extract information regarding the plant's physiological processes under the biofortification procedures. Both macro and micronutrients were mapped, providing useful insight into the posterior food processing mechanisms of this biofortified staple food. We have also shown that these kind of studies can now be performed with laboratory benchtop apparatus, rather than using synchrotron radiation, increasing the overall attractiveness of micro X-ray fluorescence in the study of highly heterogeneous biological samples.

Original languageEnglish
Pages (from-to)30-36
Number of pages7
JournalSpectrochimica Acta Part B: Atomic Spectroscopy
Publication statusPublished - 1 Jun 2016


  • Biofortification
  • Elemental mappings
  • Micro beam
  • Wheat
  • X-ray fluorescence


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