Electrophysiological and behavioural responses of the Eucalyptus weevil, Gonipterus platensis, to host plant volatiles

Sofia Branco, Eduardo P. Mateus, Marco D. R. Gomes da Silva, Davide Mendes, Sílvia Rocha, Zvi Mendel, Stefan Schütz, Maria Rosa Paiva

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Gonipterus spp. weevils (Coleoptera, Curculionidae) are pests of Eucalyptus stands in many world regions. Here, we determined the host plant volatiles that might mediate host selection behaviour in G. platensis. Eucalyptus globulus volatile organic compounds were collected by headspace solid-phase microextraction, monolithic material sorption extraction and simultaneous distillation–extraction. Gas chromatography–mass spectrometry/electroantennographic detection (GC–MS/EAD) was used to determine which compounds triggered antennal responses in the insect. Further chemical analysis of the extracted compounds was conducted using gas chromatography–mass spectrometry (GC/MS) and comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GCxGC/TOFMS). Gonipterus platensis antennal response was elicited by 51 host plant volatiles and was subsequently confirmed for 33 of these compounds using commercial standards. The behavioural response of G. platensis to individual compounds was assessed in a two-arm olfactometer with ten parallel walking chambers, coupled to video tracking and data analysis software. Weevils of known age, sex and mating status were tested. We identified eight kairomones and two allomones. The behavioural responses differed depending on insect sex and mating status. Three compounds attracted virgin females or both sexes: camphene, (+)-α-pinene and 2-phenylethanol and are potential candidates for application in integrated pest management approaches. Furthermore, the two allomones may have potential use for developing push–pull strategies.

Original languageEnglish
Pages (from-to)221-235
Number of pages15
JournalJournal Of Pest Science
Volume92
Issue number1
DOIs
Publication statusPublished - 15 Jan 2019

Fingerprint

Gonipterus
allomones
Eucalyptus
Curculionidae
host plants
gender
spectroscopy
gases
camphene
electroantennography
kairomones
insects
olfactometers
Eucalyptus globulus
volatile organic compounds
virgin females
integrated pest management
chemical analysis
walking
sorption

Keywords

  • Allomones
  • Eucalyptus globulus
  • Gas chromatography–mass spectrometry/electroantennographic detection
  • Gonipterus platensis
  • Kairomones

Cite this

@article{9badfc5b3b134b96837b2c4710e0d04b,
title = "Electrophysiological and behavioural responses of the Eucalyptus weevil, Gonipterus platensis, to host plant volatiles",
abstract = "Gonipterus spp. weevils (Coleoptera, Curculionidae) are pests of Eucalyptus stands in many world regions. Here, we determined the host plant volatiles that might mediate host selection behaviour in G. platensis. Eucalyptus globulus volatile organic compounds were collected by headspace solid-phase microextraction, monolithic material sorption extraction and simultaneous distillation–extraction. Gas chromatography–mass spectrometry/electroantennographic detection (GC–MS/EAD) was used to determine which compounds triggered antennal responses in the insect. Further chemical analysis of the extracted compounds was conducted using gas chromatography–mass spectrometry (GC/MS) and comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GCxGC/TOFMS). Gonipterus platensis antennal response was elicited by 51 host plant volatiles and was subsequently confirmed for 33 of these compounds using commercial standards. The behavioural response of G. platensis to individual compounds was assessed in a two-arm olfactometer with ten parallel walking chambers, coupled to video tracking and data analysis software. Weevils of known age, sex and mating status were tested. We identified eight kairomones and two allomones. The behavioural responses differed depending on insect sex and mating status. Three compounds attracted virgin females or both sexes: camphene, (+)-α-pinene and 2-phenylethanol and are potential candidates for application in integrated pest management approaches. Furthermore, the two allomones may have potential use for developing push–pull strategies.",
keywords = "Allomones, Eucalyptus globulus, Gas chromatography–mass spectrometry/electroantennographic detection, Gonipterus platensis, Kairomones",
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note = "This work has been supported by CENSE (Center for Environmental and Sustainability Research) which is financed by national funds from FCT/MEC (UID/AMB/04085/2013) and by the Associate Laboratory for Green Chemistry LAQV which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and cofinanced by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265).",
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Electrophysiological and behavioural responses of the Eucalyptus weevil, Gonipterus platensis, to host plant volatiles. / Branco, Sofia; Mateus, Eduardo P.; Silva, Marco D. R. Gomes da; Mendes, Davide; Rocha, Sílvia; Mendel, Zvi; Schütz, Stefan; Paiva, Maria Rosa.

In: Journal Of Pest Science, Vol. 92, No. 1, 15.01.2019, p. 221-235.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Electrophysiological and behavioural responses of the Eucalyptus weevil, Gonipterus platensis, to host plant volatiles

AU - Branco, Sofia

AU - Mateus, Eduardo P.

AU - Silva, Marco D. R. Gomes da

AU - Mendes, Davide

AU - Rocha, Sílvia

AU - Mendel, Zvi

AU - Schütz, Stefan

AU - Paiva, Maria Rosa

N1 - This work has been supported by CENSE (Center for Environmental and Sustainability Research) which is financed by national funds from FCT/MEC (UID/AMB/04085/2013) and by the Associate Laboratory for Green Chemistry LAQV which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and cofinanced by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265).

PY - 2019/1/15

Y1 - 2019/1/15

N2 - Gonipterus spp. weevils (Coleoptera, Curculionidae) are pests of Eucalyptus stands in many world regions. Here, we determined the host plant volatiles that might mediate host selection behaviour in G. platensis. Eucalyptus globulus volatile organic compounds were collected by headspace solid-phase microextraction, monolithic material sorption extraction and simultaneous distillation–extraction. Gas chromatography–mass spectrometry/electroantennographic detection (GC–MS/EAD) was used to determine which compounds triggered antennal responses in the insect. Further chemical analysis of the extracted compounds was conducted using gas chromatography–mass spectrometry (GC/MS) and comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GCxGC/TOFMS). Gonipterus platensis antennal response was elicited by 51 host plant volatiles and was subsequently confirmed for 33 of these compounds using commercial standards. The behavioural response of G. platensis to individual compounds was assessed in a two-arm olfactometer with ten parallel walking chambers, coupled to video tracking and data analysis software. Weevils of known age, sex and mating status were tested. We identified eight kairomones and two allomones. The behavioural responses differed depending on insect sex and mating status. Three compounds attracted virgin females or both sexes: camphene, (+)-α-pinene and 2-phenylethanol and are potential candidates for application in integrated pest management approaches. Furthermore, the two allomones may have potential use for developing push–pull strategies.

AB - Gonipterus spp. weevils (Coleoptera, Curculionidae) are pests of Eucalyptus stands in many world regions. Here, we determined the host plant volatiles that might mediate host selection behaviour in G. platensis. Eucalyptus globulus volatile organic compounds were collected by headspace solid-phase microextraction, monolithic material sorption extraction and simultaneous distillation–extraction. Gas chromatography–mass spectrometry/electroantennographic detection (GC–MS/EAD) was used to determine which compounds triggered antennal responses in the insect. Further chemical analysis of the extracted compounds was conducted using gas chromatography–mass spectrometry (GC/MS) and comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GCxGC/TOFMS). Gonipterus platensis antennal response was elicited by 51 host plant volatiles and was subsequently confirmed for 33 of these compounds using commercial standards. The behavioural response of G. platensis to individual compounds was assessed in a two-arm olfactometer with ten parallel walking chambers, coupled to video tracking and data analysis software. Weevils of known age, sex and mating status were tested. We identified eight kairomones and two allomones. The behavioural responses differed depending on insect sex and mating status. Three compounds attracted virgin females or both sexes: camphene, (+)-α-pinene and 2-phenylethanol and are potential candidates for application in integrated pest management approaches. Furthermore, the two allomones may have potential use for developing push–pull strategies.

KW - Allomones

KW - Eucalyptus globulus

KW - Gas chromatography–mass spectrometry/electroantennographic detection

KW - Gonipterus platensis

KW - Kairomones

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