TY - JOUR
T1 - Electrochemical Studies on Nitrite Reductase toward a Biosensor
AU - Scharf, M.
AU - Moreno, C.
AU - Costa, C.
AU - Vandijk, C.
AU - Payne, W. J.
AU - Legall, J.
AU - Moura, I.
AU - Moura, José J. G.
PY - 1995/4/26
Y1 - 1995/4/26
N2 - A c-type hexaheme nitrite reductase (NiR) isolated from nitrate-grown cells of Desulfovibrio desulfuricans (Dd) ATCC 27774 catalyses the: six-electron reduction of nitrite to ammonia. Previous electrochemical studies demonstrated that a simple electrocatalytic mechanism can be applied to this system (Moreno, C., Costa, C., Moura, I., LeGall, J., Liu, M.Y., Payne, W.J., Van Dijk, C. and Moura, J.J.G. (1992) Eur.J.Biochem.212, 79-86). Its substrate specificity, availability and stability under ambient conditions makes this enzymatic system a promising candidate for use in a biosensor device. An electrochemical study of gel-immobilized Dd NiR on a glassy carbon electrode revealed both enzymatic activity and amperometric response to nitrite. In this study it was observed that the catalytic current density is a function of the nitrite concentration in solution and follows a characteristic Michaelis-Menten-type substrate dependence. Such a biosensor device (NiR-electrode) bears the option to be used for analytical determination of nitrite in complex media.
AB - A c-type hexaheme nitrite reductase (NiR) isolated from nitrate-grown cells of Desulfovibrio desulfuricans (Dd) ATCC 27774 catalyses the: six-electron reduction of nitrite to ammonia. Previous electrochemical studies demonstrated that a simple electrocatalytic mechanism can be applied to this system (Moreno, C., Costa, C., Moura, I., LeGall, J., Liu, M.Y., Payne, W.J., Van Dijk, C. and Moura, J.J.G. (1992) Eur.J.Biochem.212, 79-86). Its substrate specificity, availability and stability under ambient conditions makes this enzymatic system a promising candidate for use in a biosensor device. An electrochemical study of gel-immobilized Dd NiR on a glassy carbon electrode revealed both enzymatic activity and amperometric response to nitrite. In this study it was observed that the catalytic current density is a function of the nitrite concentration in solution and follows a characteristic Michaelis-Menten-type substrate dependence. Such a biosensor device (NiR-electrode) bears the option to be used for analytical determination of nitrite in complex media.
UR - http://www.scopus.com/inward/record.url?scp=0029029660&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1995.1599
DO - 10.1006/bbrc.1995.1599
M3 - Article
C2 - 7733953
AN - SCOPUS:0029029660
SN - 0006-291X
VL - 209
SP - 1018
EP - 1025
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -