TY - JOUR
T1 - Electrochemical studies on c-type cytochromes at microelectrodes
AU - Correia Dos Santos, M. M.
AU - Paes De Sousa, P. M.
AU - Gonçalves, M. L. Simões
AU - Lopes, H.
AU - Moura, I.
AU - Moura, José J. G.
PY - 1999/3/19
Y1 - 1999/3/19
N2 -
The aim of this work is to use microelectrodes as a current approach for the study of unmediated electrochemistry of redox proteins. An electrochemical study of monohemic cytochromes c
552
from Pseudomonas nautica 617, cytochrome c
553
from Desulfovibrio vulgaris and horse heart cytochrome c is presented at inlaid disk microelectrodes of platinum, gold and carbon. Different electrochemical techniques were used such as linear scan, differential pulse and square wave voltammetry. The electrochemical response was also analyzed at conventional size (macro) electrodes for comparison. In all situations a promoter was used. The electrochemical behaviour was evaluated in terms of kinetics of the electrode processes and the formal potentials determined. Diffusion coefficients were also calculated from the voltammetric data. A critical comparison of the results obtained is carried out and the advantages of microelectrodes for electrochemical studies of metalloproteins are pointed out.
AB -
The aim of this work is to use microelectrodes as a current approach for the study of unmediated electrochemistry of redox proteins. An electrochemical study of monohemic cytochromes c
552
from Pseudomonas nautica 617, cytochrome c
553
from Desulfovibrio vulgaris and horse heart cytochrome c is presented at inlaid disk microelectrodes of platinum, gold and carbon. Different electrochemical techniques were used such as linear scan, differential pulse and square wave voltammetry. The electrochemical response was also analyzed at conventional size (macro) electrodes for comparison. In all situations a promoter was used. The electrochemical behaviour was evaluated in terms of kinetics of the electrode processes and the formal potentials determined. Diffusion coefficients were also calculated from the voltammetric data. A critical comparison of the results obtained is carried out and the advantages of microelectrodes for electrochemical studies of metalloproteins are pointed out.
UR - http://www.scopus.com/inward/record.url?scp=0344286077&partnerID=8YFLogxK
U2 - 10.1016/S0022-0728(98)00474-4
DO - 10.1016/S0022-0728(98)00474-4
M3 - Article
AN - SCOPUS:0344286077
SN - 0022-0728
VL - 464
SP - 76
EP - 84
JO - Journal Of Electroanalytical Chemistry
JF - Journal Of Electroanalytical Chemistry
IS - 1
ER -