Electrochemical properties of pH-dependent flavocytochrome c3 from Shewanella putrefaciens adsorbed onto unmodified and catechol-modified edge plane pyrolytic graphite electrode

Estelle Lebègue, Nazua L. Costa, Bruno M. Fonseca, Ricardo O. Louro, Frédéric Barrière

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The electroactivity of adsorbed flavocytochrome c3, a tetraheme FAD-containing flavoenzyme isolated from the bacterium Shewanella putrefaciens, is investigated by cyclic voltammetry at an edge plane pyrolytic graphite electrode before and after modification with grafted catechol serving as an efficient pH sensor based on a redox readout. Flavocytochrome c3 adsorption onto the unmodified or modified electrode surface is successfully achieved by cyclic voltammetry (100 consecutive cycles) in a flavocytochrome c3 solution containing polymyxin as co-adsorbate. The immobilized flavocytochrome c3 retains its electrochemical activity and its catalytic fumarate reductase activity. The redox activity of the protein arises from its FAD and four hemes cofactors. The experiments evidence that the hemes' redox potential of flavocytochrome c3 from Shewanella putrefaciens, for which no crystal structure is yet available, depend on pH which is at variance with data from the other strains Shewanella frigidimarina or Shewanella oneidensis.

Original languageEnglish
Article number113232
JournalJournal Of Electroanalytical Chemistry
Publication statusPublished - 15 Aug 2019



  • Catechol pH probe
  • Cyclic voltammetry
  • Electrode surface modification
  • Flavocytochrome c
  • Fumarate reductase
  • pH-dependent redox activity
  • Shewanella putrefaciens

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