Electrochemical biosensor based on biomimetic material for myoglobin detection

Felismina T. C. Moreira, Rosa A. F. Dutra, João Paulo da Costa de Noronha, M. Goreti F Sales

Research output: Contribution to journalArticle

56 Citations (Scopus)


A novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVC COOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FTIR) studies confirmed the surface modification. The MIP/Au-SPE devices displayed a linear behaviour in EIS from 0.852 to 4.26 g mL−1, of positive slope 6.50 ± 1.48 (k mL g−1). The limit of detection was 2.25 g mL−1. Square wave voltammetric (SWV) assays were made in parallel and showed linear responses between 1.1 and 2.98 g mL−1. A current decrease was observed against Myo concentration, producing average slopes of −0.28 ± 0.038 A mL g−1. MIP/Au-SPE also showed good results in terms of selectivity. The error% found for each interfering species were 7% for troponin T (TnT), 11% for bovine serum albumin (BSA) and 2% for creatine kinase MB (CKMB), respectively. Overall, the technical modification over the Au-SPE was found a suitable approach for screening Myo in biological fluids.
Original languageEnglish
Pages (from-to)481-487
JournalElectrochimica Acta
Publication statusPublished - 30 Sep 2013


  • Biosensor
  • Myoglobin
  • Screen printed electrodes
  • Sensor
  • Surface molecular imprint

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