Electrochemical Actuation of a DyP Peroxidase: A Facile Method for Drastic Improvement of the Catalytic Performance

Magalí F. Scocozza, Francisco Vieyra, Fernando Battaglini, Ligia O. Martins, Daniel H. Murgida

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Dye decolorizing peroxidases (DyP) have attracted interest for applications such as dye-containing wastewater remediation and biomass processing. So far, efforts to improve operational pH ranges, activities, and stabilities have focused on site-directed mutagenesis and directed evolution strategies. Here, we show that the performance of the DyP from Bacillus subtilis can be drastically boosted without the need for complex molecular biology procedures by simply activating the enzyme electrochemically in the absence of externally added H2O2. Under these conditions, the enzyme shows specific activities toward a variety of chemically different substrates that are significantly higher than in its canonical operation. Moreover, it presents much broader pH activity profiles with the maxima shifted toward neutral to alkaline. We also show that the enzyme can be successfully immobilized on biocompatible electrodes. When actuated electrochemically, the enzymatic electrodes have two orders of magnitude higher turnover numbers than with the standard H2O2-dependent operation and preserve about 30% of the initial electrocatalytic activity after 5 days of operation-storage cycles.

Original languageEnglish
Pages (from-to)7437-7449
Number of pages13
JournalACS Catalysis
Volume13
Issue number11
DOIs
Publication statusPublished - 2 Jun 2023

Keywords

  • dye decolorizing peroxidases
  • electrocatalysis
  • heme peroxidases
  • hydroxyl radical
  • ligninolytic enzymes
  • superoxide

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