@article{1e60c9a2e2434b1ebfcf8ec2ec0f5c06,
title = "Electro-enzymatic ATP regeneration coupled to biocatalytic phosphorylation reactions",
abstract = "Adenosine-5-triphosphate (ATP) is the main energy vector in biological systems, thus its regeneration is an important issue for the application of many enzymes of interest in biocatalysis and synthetic biology. We have developed an electroenzymatic ATP regeneration system consisting in a gold electrode modified with a floating phospholipid bilayer that allows coupling the catalytic activity of two membrane-bound enzymes: NiFeSe hydrogenase from Desulfovibrio vulgaris and F1Fo-ATP synthase from Escherichia coli. Thus, H2 is used as a fuel for producing ATP. This electro-enzymatic assembly is studied as ATP regeneration system of phosphorylation reactions catalysed by kinases, such as hexokinase and NAD+-kinase for respectively producing glucose-6-phosphate and NADP+.",
keywords = "ATP regeneration, ATP-synthase, Hydrogenase, Kinase, Phosphorylation",
author = "Gabriel Garc{\'i}a-Molina and Paolo Natale and Coito, {Ana M.} and Cava, {Daniel G.} and {A. C. Pereira}, In{\'e}s and Iv{\'a}n L{\'o}pez-Montero and Marisela V{\'e}lez and Marcos Pita and {De Lacey}, {Antonio L.}",
note = "Funding Information: A.L.D, M.P. and M.V. thank grants RTI2018-095090-B-I00 and PID2021-1241160B-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by the European Union, and 2021AEP014 funded by CSIC. G.G.M. thanks grant BES-2016-078815 funded by MCIN/AEI/ 10.13039/501100011033 and by the European Union. I.L.-M. and M.V. acknowledge financial support through grant S2018/BAA-4403 SINOXPHOS-CM (EU-FEDER). I.A.C.P. and A.M.C. thank support from the Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia through fellowship SFRH/BD/146475/2019 and MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = aug,
doi = "10.1016/j.bioelechem.2023.108432",
language = "English",
volume = "152",
journal = "Bioelectrochemistry",
issn = "1567-5394",
publisher = "Elsevier Science B.V., Amsterdam.",
}