Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform

Lígia C. Gomes-Da-Silva, Yolanda Fernández, Ibane Abasolo, Simo Schwartz, José S. Ramalho, Maria C. Pedroso De Lima, Sérgio Simões, João N. Moreira

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aim: The design of novel F3-targeted liposomes with adequate features for systemic administration, to enable efficient intracellular delivery of siRNA toward both cancer and endothelial cells from angiogenic blood vessels. Materials & methods: Cellular association studies were performed by flow cytometry. Gene silencing was evaluated with eGFP-overexpressing cells, by flow cytometry and real-time reverse-transcription PCR. Safety and immunogenicity was assessed in CD1 mice. Results: A strong improvement on siRNA internalization by the target cells was achieved, which was correlated with effective downregulation of eGFP. In addition, the F3-targeted liposomes were nonimmunogenic, even in a multiadministration schedule. Conclusion: Overall, the developed F3-targeted nanocarrier constitutes a valuable tool for the specific and safe systemic delivery of siRNA to solid tumors. Original submitted 19 April 2012; Revised submitted 12 September 2012; Published online 8 February 201.

Original languageEnglish
Pages (from-to)1397-1413
Number of pages17
JournalNanomedicine
Volume8
Issue number9
DOIs
Publication statusPublished - Sep 2013

Keywords

  • blood stability
  • cancer
  • dual-targeted delivery
  • immunogenicity
  • intravenous administration
  • pH-sensitive liposome
  • siRNA

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    Gomes-Da-Silva, L. C., Fernández, Y., Abasolo, I., Schwartz, S., Ramalho, J. S., Pedroso De Lima, M. C., ... Moreira, J. N. (2013). Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform. Nanomedicine, 8(9), 1397-1413. https://doi.org/10.2217/nnm.12.174