Effects of permethrin exposure on antioxidant enzymes and protein status in Mediterranean clams Ruditapes decussatus

Badreddine Sellami, Hela Louati, Mohamed Dellali, Patricia Aissa, Ezzeddine Mahmoudi, Ana Maria Coelho, David Sheehan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effects of permethrin (PER) on a panel of antoxidant enzymes; superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and indices of protein oxidation status (carbonylation and free thiols) were determined in digestive gland and gills of the clam Ruditapes decussatus. Animals were exposed to 100 ppb PER for 2 days. These enzyme activities increased significantly in digestive gland (p <0.05) after PER treatment and oxidative modification of proteins was detected in both gill and digestive gland extracts using redox proteomics. PER exposure significantly reduced the amount of protein free thiol groups in digestive gland rather than in gill, when compared to controls. Conversely, digestive gland showed significantly higher levels of carbonylated proteins than gill after PER exposure. Some proteins were successfully identified by mass spectrometry of tryptic peptides. Our data suggest that digestive gland of R. decussatus can be used as a model tissue for investigating environmental risk of PER contamination.

Original languageEnglish
Pages (from-to)4461-4472
Number of pages12
JournalEnvironmental Science and Pollution Research
Volume21
Issue number6
DOIs
Publication statusPublished - 2014

Keywords

  • Oxidative stress
  • Permethrin
  • Redox proteomics
  • Ruditapes decussatus

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