Effects of elevated carbon dioxide on the hematological parameters of a temperate catshark

Maria Rita Pegado, Catarina P. Santos, Marta S. Pimentel, Ricardo Cyrne, Maria Paulo, Ana Luísa Maulvault, Dayanne Raffoul, Mário Diniz, R. Bispo, Rui Rosa

Research output: Contribution to journalArticle

Abstract

Atmospheric CO2 levels have been rising due to an increase in anthropic activities and its implications over marine ecosystems are unprecedented. The present study focused on the effects of ocean acidification (OA) on key hematological parameters of the juvenile small‐spotted catsharks (Scyliorhinus canicula). Eggs were reared throughout the entire embryogenesis (~4 months) plus 5 additional months, in two experimental treatments (control: pCO2 ~ 400 μatm; and high CO2: pCO2 ~ 900 μatm, Δ −0.3 pH units). After blood collection, the following hematological parameters were evaluated: (a) normal blood cells count (erythrocytes, leukocytes, and thrombocytes), (b) presence of erythrocytes with nuclear abnormalities, and (c) erythrocyte nucleus to cytoplasmic ratio. Concomitantly, to determine the cardiac and hematopoietic conditions, the spleen and heart to body ratios were also assessed. The present findings indicate that the measured variables may not be affected by elevated pCO2 in this temperate species, as no significant differences were observed between treatments across all the endpoints tested. Nonetheless, it is worth mentioning a decreasing trend observed in a number of thrombocytes associated with OA, which should foster further investigation, regarding other aspects of their coagulation response. Along with OA, other stressors are expected to impact marine life, such as warming and hypoxia. Thus, future research should aim to investigate the cumulative effect of these stressors on hematological parameters in sharks.
Original languageEnglish
JournalJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
DOIs
Publication statusE-pub ahead of print - 3 Dec 2019

Fingerprint

blood platelets
erythrocytes
carbon dioxide
Scyliorhinus canicula
blood cell counts
coagulation
endpoints
sharks
hypoxia
leukocytes
spleen
embryogenesis
heart
blood
ocean acidification
marine ecosystems

Keywords

  • early stages
  • elasmobranch
  • hematology
  • ocean acidification
  • Scyliorhinus canicula
  • shark

Cite this

@article{b06fee4c0b274c27a9a5c6940e532370,
title = "Effects of elevated carbon dioxide on the hematological parameters of a temperate catshark",
abstract = "Atmospheric CO2 levels have been rising due to an increase in anthropic activities and its implications over marine ecosystems are unprecedented. The present study focused on the effects of ocean acidification (OA) on key hematological parameters of the juvenile small‐spotted catsharks (Scyliorhinus canicula). Eggs were reared throughout the entire embryogenesis (~4 months) plus 5 additional months, in two experimental treatments (control: pCO2 ~ 400 μatm; and high CO2: pCO2 ~ 900 μatm, Δ −0.3 pH units). After blood collection, the following hematological parameters were evaluated: (a) normal blood cells count (erythrocytes, leukocytes, and thrombocytes), (b) presence of erythrocytes with nuclear abnormalities, and (c) erythrocyte nucleus to cytoplasmic ratio. Concomitantly, to determine the cardiac and hematopoietic conditions, the spleen and heart to body ratios were also assessed. The present findings indicate that the measured variables may not be affected by elevated pCO2 in this temperate species, as no significant differences were observed between treatments across all the endpoints tested. Nonetheless, it is worth mentioning a decreasing trend observed in a number of thrombocytes associated with OA, which should foster further investigation, regarding other aspects of their coagulation response. Along with OA, other stressors are expected to impact marine life, such as warming and hypoxia. Thus, future research should aim to investigate the cumulative effect of these stressors on hematological parameters in sharks.",
keywords = "early stages, elasmobranch, hematology, ocean acidification, Scyliorhinus canicula, shark",
author = "Pegado, {Maria Rita} and Santos, {Catarina P.} and Pimentel, {Marta S.} and Ricardo Cyrne and Maria Paulo and Maulvault, {Ana Lu{\'i}sa} and Dayanne Raffoul and M{\'a}rio Diniz and R. Bispo and Rui Rosa",
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language = "English",
journal = "Journal of Experimental Zoology Part A: Ecological Genetics and Physiology",

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Effects of elevated carbon dioxide on the hematological parameters of a temperate catshark. / Pegado, Maria Rita; Santos, Catarina P.; Pimentel, Marta S.; Cyrne, Ricardo ; Paulo, Maria; Maulvault, Ana Luísa; Raffoul, Dayanne ; Diniz, Mário; Bispo, R.; Rosa, Rui.

In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology , 03.12.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of elevated carbon dioxide on the hematological parameters of a temperate catshark

AU - Pegado, Maria Rita

AU - Santos, Catarina P.

AU - Pimentel, Marta S.

AU - Cyrne, Ricardo

AU - Paulo, Maria

AU - Maulvault, Ana Luísa

AU - Raffoul, Dayanne

AU - Diniz, Mário

AU - Bispo, R.

AU - Rosa, Rui

N1 - Fundação para a Ciência e a Tecnologia. Grant Numbers: SFRH/BD/111691/2015, SFRH/BD/117890/2016, SFRH/BPD/117533/2016, UID/MAR/04292/2019, UID/Multi/04378/2019, PTDC/AAG‐GLO/1926/2014. MAR2020. Grant Number: MAR‐01.04.02‐FEAMP‐0006.

PY - 2019/12/3

Y1 - 2019/12/3

N2 - Atmospheric CO2 levels have been rising due to an increase in anthropic activities and its implications over marine ecosystems are unprecedented. The present study focused on the effects of ocean acidification (OA) on key hematological parameters of the juvenile small‐spotted catsharks (Scyliorhinus canicula). Eggs were reared throughout the entire embryogenesis (~4 months) plus 5 additional months, in two experimental treatments (control: pCO2 ~ 400 μatm; and high CO2: pCO2 ~ 900 μatm, Δ −0.3 pH units). After blood collection, the following hematological parameters were evaluated: (a) normal blood cells count (erythrocytes, leukocytes, and thrombocytes), (b) presence of erythrocytes with nuclear abnormalities, and (c) erythrocyte nucleus to cytoplasmic ratio. Concomitantly, to determine the cardiac and hematopoietic conditions, the spleen and heart to body ratios were also assessed. The present findings indicate that the measured variables may not be affected by elevated pCO2 in this temperate species, as no significant differences were observed between treatments across all the endpoints tested. Nonetheless, it is worth mentioning a decreasing trend observed in a number of thrombocytes associated with OA, which should foster further investigation, regarding other aspects of their coagulation response. Along with OA, other stressors are expected to impact marine life, such as warming and hypoxia. Thus, future research should aim to investigate the cumulative effect of these stressors on hematological parameters in sharks.

AB - Atmospheric CO2 levels have been rising due to an increase in anthropic activities and its implications over marine ecosystems are unprecedented. The present study focused on the effects of ocean acidification (OA) on key hematological parameters of the juvenile small‐spotted catsharks (Scyliorhinus canicula). Eggs were reared throughout the entire embryogenesis (~4 months) plus 5 additional months, in two experimental treatments (control: pCO2 ~ 400 μatm; and high CO2: pCO2 ~ 900 μatm, Δ −0.3 pH units). After blood collection, the following hematological parameters were evaluated: (a) normal blood cells count (erythrocytes, leukocytes, and thrombocytes), (b) presence of erythrocytes with nuclear abnormalities, and (c) erythrocyte nucleus to cytoplasmic ratio. Concomitantly, to determine the cardiac and hematopoietic conditions, the spleen and heart to body ratios were also assessed. The present findings indicate that the measured variables may not be affected by elevated pCO2 in this temperate species, as no significant differences were observed between treatments across all the endpoints tested. Nonetheless, it is worth mentioning a decreasing trend observed in a number of thrombocytes associated with OA, which should foster further investigation, regarding other aspects of their coagulation response. Along with OA, other stressors are expected to impact marine life, such as warming and hypoxia. Thus, future research should aim to investigate the cumulative effect of these stressors on hematological parameters in sharks.

KW - early stages

KW - elasmobranch

KW - hematology

KW - ocean acidification

KW - Scyliorhinus canicula

KW - shark

U2 - 10.1002/jez.2333

DO - 10.1002/jez.2333

M3 - Article

JO - Journal of Experimental Zoology Part A: Ecological Genetics and Physiology

JF - Journal of Experimental Zoology Part A: Ecological Genetics and Physiology

ER -