Effects of climate change on marine organisms: A proteomic approach

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The effects of climate change, namely sea warming and ocean acidification, cause changes in the physiology, phenology, and biogeographical distribution of organisms. The stress induced in marine organisms at the proteome level, altering the expression of proteins and their modifications, is still largely unknown. The most common techniques used in marine proteomics regarding climate change are 2D electrophoresis and protein identification by mass spectrometry. Typically, the workflow includes several sequential steps: (1) sample preparation, (2) protein denaturation and reduction, (3) protein (peptide) separation, enzymatic digestion, and mass spectrometry analysis, and (4) bioinformatics and protein identification. As such the “omics”, and particularly proteomics, have the potential to provide new insights into the integrative functional responses of organisms to environmental stresses. Studies developed to date on proteome changes suggest a convergence toward a common set of stress-induced proteins related to metabolism, the cytoskeleton, chaperones, and diverse protective proteins. Therefore, this chapter discusses the main aspects related to climate change that affect marine organisms, with a special emphasis on the typical proteomic workflow used for marine organisms, the most relevant studies, the main problems, and future challenges.

Original languageEnglish
Title of host publicationEnvironmental Problems in Marine Biology
Subtitle of host publicationMethodological Aspects and Applications
PublisherCRC Press
Pages190-212
Number of pages23
ISBN (Electronic)9781482264517
ISBN (Print)9781482264500
DOIs
Publication statusPublished - 1 Jan 2017

Fingerprint

Aquatic Organisms
proteomics
Climate Change
Climate change
Proteomics
climate change
protein
organisms
Workflow
Proteome
proteome
Proteins
proteins
Mass Spectrometry
mass spectrometry
Protein Denaturation
Mass spectrometry
Heat-Shock Proteins
cytoskeleton
Computational Biology

Cite this

Diniz, M., Madeira, D., & Araújo, E. (2017). Effects of climate change on marine organisms: A proteomic approach. In Environmental Problems in Marine Biology: Methodological Aspects and Applications (pp. 190-212). CRC Press. https://doi.org/10.1201/9781315119113
Diniz, Mário ; Madeira, Diana ; Araújo, Eduardo. / Effects of climate change on marine organisms : A proteomic approach. Environmental Problems in Marine Biology: Methodological Aspects and Applications. CRC Press, 2017. pp. 190-212
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Diniz, M, Madeira, D & Araújo, E 2017, Effects of climate change on marine organisms: A proteomic approach. in Environmental Problems in Marine Biology: Methodological Aspects and Applications. CRC Press, pp. 190-212. https://doi.org/10.1201/9781315119113

Effects of climate change on marine organisms : A proteomic approach. / Diniz, Mário; Madeira, Diana; Araújo, Eduardo.

Environmental Problems in Marine Biology: Methodological Aspects and Applications. CRC Press, 2017. p. 190-212.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Diniz M, Madeira D, Araújo E. Effects of climate change on marine organisms: A proteomic approach. In Environmental Problems in Marine Biology: Methodological Aspects and Applications. CRC Press. 2017. p. 190-212 https://doi.org/10.1201/9781315119113