Effect of UV radiation on DPPG and DMPC liposomes in presence of catechin molecules

Filipa Pires, Gonçalo Magalhães-Mota, Paulo António Ribeiro, Maria Raposo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Catechin molecules are known to reduce the oxidative stress-induced by radiation acting as scavenger of the reactive oxygen species, preventing in this way the damage in biomolecules. In this work, the effect of radiation on liposomes of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)(sodium salt) (DPPG) and of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) is analyzed in the absence and presence of epigallocatechin-3-gallate (EGCG) molecules, having in view the evaluation of the photosensitizing properties and the efficacy of these molecules to modulate cell membrane damage mechanisms. The obtained results demonstrate that the damage by UV radiation on DPPG and DMPC liposomes is strongly dependent of the presence of EGCG molecules. While DPPG liposomes are protected from radiation in presence of EGCG, the EGCG molecules are damaged by the radiation supporting the idea that EGCG are strongly adsorbed on the inner and outer liposome surfaces due hydrogen bonding. This suggests that EGCG molecules in the inner surface can be protected from radiation. In the case of DMPC liposomes, the EGCG molecules are affected by radiation as well as the DMPC molecules. This is explained if the EGCG chroman group is positioned between DMPC lipids while the gallic acid groups float over the liposomes.

Original languageEnglish
Title of host publicationComputational Intelligence Methods for Bioinformatics and Biostatistics - 13th International Meeting, CIBB 2016, Revised Selected Papers
PublisherSpringer Verlag
Pages172-183
Number of pages12
Volume10477 LNBI
ISBN (Print)9783319678337
DOIs
Publication statusPublished - 2017
Event13th International Meeting on Computational Intelligence Methods for Bioinformatics and Biostatistics, CIBB 2016 - Stirling, United Kingdom
Duration: 1 Sep 20163 Sep 2016

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
Volume10477 LNBI
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Conference

Conference13th International Meeting on Computational Intelligence Methods for Bioinformatics and Biostatistics, CIBB 2016
CountryUnited Kingdom
CityStirling
Period1/09/163/09/16

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Keywords

  • Cell membrane
  • Cellular detoxification
  • Delivery system
  • Natural antioxidant
  • Physical interactions

Cite this

Pires, F., Magalhães-Mota, G., Ribeiro, P. A., & Raposo, M. (2017). Effect of UV radiation on DPPG and DMPC liposomes in presence of catechin molecules. In Computational Intelligence Methods for Bioinformatics and Biostatistics - 13th International Meeting, CIBB 2016, Revised Selected Papers (Vol. 10477 LNBI, pp. 172-183). (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); Vol. 10477 LNBI). Springer Verlag. https://doi.org/10.1007/978-3-319-67834-4_14