TY - GEN
T1 - Effect of UV radiation on DPPG and DMPC liposomes in presence of catechin molecules
AU - Pires, Filipa
AU - Magalhães-Mota, Gonçalo
AU - Ribeiro, Paulo António
AU - Raposo, Maria
N1 - Sem PDF.
PY - 2017
Y1 - 2017
N2 - Catechin molecules are known to reduce the oxidative stress-induced by radiation acting as scavenger of the reactive oxygen species, preventing in this way the damage in biomolecules. In this work, the effect of radiation on liposomes of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)(sodium salt) (DPPG) and of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) is analyzed in the absence and presence of epigallocatechin-3-gallate (EGCG) molecules, having in view the evaluation of the photosensitizing properties and the efficacy of these molecules to modulate cell membrane damage mechanisms. The obtained results demonstrate that the damage by UV radiation on DPPG and DMPC liposomes is strongly dependent of the presence of EGCG molecules. While DPPG liposomes are protected from radiation in presence of EGCG, the EGCG molecules are damaged by the radiation supporting the idea that EGCG are strongly adsorbed on the inner and outer liposome surfaces due hydrogen bonding. This suggests that EGCG molecules in the inner surface can be protected from radiation. In the case of DMPC liposomes, the EGCG molecules are affected by radiation as well as the DMPC molecules. This is explained if the EGCG chroman group is positioned between DMPC lipids while the gallic acid groups float over the liposomes.
AB - Catechin molecules are known to reduce the oxidative stress-induced by radiation acting as scavenger of the reactive oxygen species, preventing in this way the damage in biomolecules. In this work, the effect of radiation on liposomes of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)(sodium salt) (DPPG) and of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) is analyzed in the absence and presence of epigallocatechin-3-gallate (EGCG) molecules, having in view the evaluation of the photosensitizing properties and the efficacy of these molecules to modulate cell membrane damage mechanisms. The obtained results demonstrate that the damage by UV radiation on DPPG and DMPC liposomes is strongly dependent of the presence of EGCG molecules. While DPPG liposomes are protected from radiation in presence of EGCG, the EGCG molecules are damaged by the radiation supporting the idea that EGCG are strongly adsorbed on the inner and outer liposome surfaces due hydrogen bonding. This suggests that EGCG molecules in the inner surface can be protected from radiation. In the case of DMPC liposomes, the EGCG molecules are affected by radiation as well as the DMPC molecules. This is explained if the EGCG chroman group is positioned between DMPC lipids while the gallic acid groups float over the liposomes.
KW - Cell membrane
KW - Cellular detoxification
KW - Delivery system
KW - Natural antioxidant
KW - Physical interactions
UR - http://www.scopus.com/inward/record.url?scp=85032700760&partnerID=8YFLogxK
U2 - 10.1007/978-3-319-67834-4_14
DO - 10.1007/978-3-319-67834-4_14
M3 - Conference contribution
AN - SCOPUS:85032700760
SN - 9783319678337
VL - 10477 LNBI
T3 - Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
SP - 172
EP - 183
BT - Computational Intelligence Methods for Bioinformatics and Biostatistics - 13th International Meeting, CIBB 2016, Revised Selected Papers
PB - Springer Verlag
T2 - 13th International Meeting on Computational Intelligence Methods for Bioinformatics and Biostatistics, CIBB 2016
Y2 - 1 September 2016 through 3 September 2016
ER -