Effect of synthesized inhibitors on Babesipain-1, a new cysteine protease from the bovine piroplasm Babesia bigemina

T. M. Martins, Lídia M. D. Gonçalves, R. Capela, Rui Moreira, V. E. do Rosário, A. Domingos

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Papain-like cysteine proteases (CP) have been shown to have essential roles in parasitic protozoa and are under study as promising drug targets. One gene was identified by sequence similarity search to be homologous to the CP family in the ongoing Babesia bigemina genome sequencing project database. The newly identified CP gene, called babesipain-1, was cloned and expressed as a fusion protein, and the effect of different inhibitors on proteolytic activity was tested. A series of new artemisinin-vinyl sulfone hybrid molecules were tested as inhibitors being effective on the range of 0.3-30 lμ, depending on the corecontaining molecule.

Original languageEnglish
Pages (from-to)68-69
Number of pages2
JournalTransboundary and Emerging Diseases
Volume57
Issue number1-2
DOIs
Publication statusPublished - Apr 2010

Keywords

  • Babesia
  • Babesipain-1
  • Cysteine proteases
  • Inhibition studies
  • Recombinant expression

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