Effect of ScCO2 on the decontamination of PECs-based cryogels: A comparison with H2O steam and H2O2 nebulization methods

Cristiana S. A. Bento, Henrique Carrêlo, Susana Alarico, Nuno Empadinhas, Hermínio C. de Sousa, Maria Teresa Cidade, Mara E. M. Braga

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Abstract

Biopolymers present ideal properties to be used in wound dressing solutions. By mixing two oppositely charged macromolecules it is possible to form polyelectrolyte complex (PEC) based cryogels using lyophilization. Their application in the biomedical field is limited due to their sterilization requirements, as conventional methods compromise their physicochemical properties. ScCO2 appears as an alternative method for decontamination. This work assessed several cryogel PEC formulations, chitosan-pectin, gelatine-xanthan gum and alginate-gelatine. PEC formation was confirmed by FTIR and rheological analysis. While steam sterilization compromised cryogels’ chemical and morphological properties, decontamination with scCO2 proved to be a promising method for decontamination of PEC-cryogels, because, similarly to what is observed with hydrogen peroxide, it does not compromise their physicochemical properties.
Original languageEnglish
Article number123451
Number of pages14
JournalInternational Journal of Pharmaceutics
Volume646
DOIs
Publication statusPublished - 5 Nov 2023

Keywords

  • Decontamination
  • HO steam
  • HO nebulization
  • Natural polymers
  • PECs-based cryogels
  • scCO
  • Wound dressing

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