@article{837bfec216f244378e42a54f2cc960c2,
title = "Effect of pH on the thermostability and redox properties of cytochrome c552 from Wolinella succinogenes",
abstract = "Cytochrome c552 from Wolinella succinogenes is one of the few examples of a low reduction potential class I c-type cytochrome with a mixture of high/low spin state populations observed in its visible spectrum. Analysis of its structural model suggests that the heme is Met/His coordinated and highly solvent-exposed. This supports the hypothesis that it is the solvent accessibility of the propionate groups that controls the reduction potential of small c-type cytochromes. The visible spectra obtained at different pH values reveal the presence of a protonable group with a pKa of 7.3, which also influences the reduction potential of this small cytochrome c552 (Em 0' of 97 ± 5 mV, pH 7.0) and can be either an H2O/OH -group distantly coordinating the heme iron, or one of the propionate groups. The thermostability of cytochrome c552 has been studied by circular dichroism and differential scanning calorimetry, indicating a highly stable protein at pH 5-7 (90 ºC to 77 ºC).",
keywords = "cytochrome c, Wolinella succinogenes, pH effect, thermostability, circular dichroism, differential scanning calorimetry, cyclic voltammetry",
author = "Mordido, {Vitor H.} and Carepo, {Marta S. P.} and Cordas, {Cristina M.} and Navendu Paul and J{\"o}rg Simon and Isabel Moura and Pauleta, {Sofia R.}",
note = "info:eu-repo/grantAgreement/FCT/Concurso de Projetos de I&D em Todos os Dom{\'i}nios Cient{\'i}ficos - 2022/2022.01152.PTDC/PT# info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT# info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT# info:eu-repo/grantAgreement/FCT/Concurso para Atribui{\c c}{\~a}o do Estatuto e Financiamento de Laborat{\'o}rios Associados (LA)/LA%2FP%2F0140%2F2020/PT# info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT# info:eu-repo/grantAgreement/FCT/Concurso de avalia{\c c}{\~a}o no {\^a}mbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Program{\'a}tico/UIDP%2F50006%2F2020/PT# info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0008%2F2020/PT# Acknowledgments The authors acknowledge the Biolab for the acquisition of CD and DSC data, and Hugo Santos and the BIOSCOPE group for determining the molecular mass by LC-ESI-MS of the isolated Ws cytochrome",
year = "2024",
month = may,
day = "22",
doi = "10.3389/fchbi.2024.1398105",
language = "English",
volume = "3",
journal = "Frontiers in Chemical Biology",
issn = "2813-530X",
publisher = "Frontiers Media",
}
