TY - JOUR
T1 - Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus
T2 - evaluation of two ozonation devices
AU - Caetano, Maicon Henrique
AU - Ferreira, Adriano Menis
AU - de Andrade, Denise
AU - Lopes de Sousa, Álvaro Francisco
AU - Carneiro, Liliane Moretti
AU - Barbosa, Alessandra Lyrio
AU - Félix de Carvalho, Herica Emilia
AU - Rigotti, Marcelo Alessandro
AU - Gottardo de Almeida, Margarete Teresa
N1 - Funding Information:
We would like to thank Dr. Fernando Ferrari for carrying out the statistical analyses. This study was financed in part by the Coordena\u00E7\u00E3o de Aperfei\u00E7oamento de Pessoal de N\u00EDvel Superior \u2013 Brazil (CAPES) \u2013 Finance Code 001, Universidade Federal de Mato Grosso do Sul-UFMS/MEC, Brazil, and National Council for Scientific and Technological Development \u2013 CNPq Brazil.
Publisher Copyright:
Copyright © 2024 Caetano et al.
PY - 2024/3
Y1 - 2024/3
N2 - Introduction: Candida albicans and Aspergillus fumigatus are two important agents of Healthcare-associated infections. This study aimed to evaluate the antifungal activity of ozone (O3) gas produced by two commercial devices against cultures of these two species. Methodology: Sterile plastic plates were inoculated with C. albicans and A. fumigatus and placed on a countertop at three distances (30 cm, 1 m, and 2 m) and three positions in relation to the wall (near, middle, and away), considering the source of O3. Plates were exposed to O3 for one hour and incubated. After incubation, the counting of colony-forming units was performed. As a control, an inoculated plate was incubated, without being exposed to O3. Tests were carried out with two different devices (namely, Mod.I and Mod.II), with the air conditioner on and off, in triplicate. Results: Both devices showed antifungal activity. Mod. I presented better results, due to a higher flow rate. The best activity was on plates at 30 cm, middle position. Contrarily, on plates at 2m, near the wall, the inhibition activity was lower. The best results were obtained with the air conditioner off. Candida albicans was more sensitive to O3 than A. fumigatus. Conclusions: This method of decontamination by O3 gas shows potential due to its fast and easy execution. The establishment of new protocols for hygiene and hospital disinfection using this approach should be considered, which may reduce environmental contamination by fungi and, consequently, the burden of fungal infections.
AB - Introduction: Candida albicans and Aspergillus fumigatus are two important agents of Healthcare-associated infections. This study aimed to evaluate the antifungal activity of ozone (O3) gas produced by two commercial devices against cultures of these two species. Methodology: Sterile plastic plates were inoculated with C. albicans and A. fumigatus and placed on a countertop at three distances (30 cm, 1 m, and 2 m) and three positions in relation to the wall (near, middle, and away), considering the source of O3. Plates were exposed to O3 for one hour and incubated. After incubation, the counting of colony-forming units was performed. As a control, an inoculated plate was incubated, without being exposed to O3. Tests were carried out with two different devices (namely, Mod.I and Mod.II), with the air conditioner on and off, in triplicate. Results: Both devices showed antifungal activity. Mod. I presented better results, due to a higher flow rate. The best activity was on plates at 30 cm, middle position. Contrarily, on plates at 2m, near the wall, the inhibition activity was lower. The best results were obtained with the air conditioner off. Candida albicans was more sensitive to O3 than A. fumigatus. Conclusions: This method of decontamination by O3 gas shows potential due to its fast and easy execution. The establishment of new protocols for hygiene and hospital disinfection using this approach should be considered, which may reduce environmental contamination by fungi and, consequently, the burden of fungal infections.
KW - Aspergillus fumigatus
KW - Candida albicans
KW - disinfection
KW - ozonation
KW - Ozone
UR - http://www.scopus.com/inward/record.url?scp=85190989035&partnerID=8YFLogxK
U2 - 10.3855/jidc.15344
DO - 10.3855/jidc.15344
M3 - Article
C2 - 38635625
AN - SCOPUS:85190989035
SN - 2036-6590
VL - 18
SP - 473
EP - 479
JO - Journal Of Infection In Developing Countries
JF - Journal Of Infection In Developing Countries
IS - 3
ER -