Effect of handling, confinement and crowding in HSP70 production in Pachygrapsus marmoratus, a model species for climate change experiments

The aim of this study was to investigate the effect of handling, con in an intertidal crab. HSP70 has been widely used as a biochemical indicator of thermal stress; however studies on the effect of experimental conditions and handling on HSP70 expression are very scarce. The response of marbled crab, through an experiment in captivity, where 42 juveniles (10 an experimental container (60×42×10 cm) and exposed to three treatments: 1) increasing temperatures and periodic handling, 2) only to periodic handling and 3) no handling, for 12 h. The objective of this work was to investigate whether the HSP70 production measured in an increasing temperature experiment is a response to temperature alone or whether manipulation, con also increase HSP70 production. Three individuals were sampled after t=0, t=4, t=6, t=8, t=10 and t=12 h, for the quanti that while temperature has a signi con and handling are not parasitic variables in experiments with this species considering increasing temperatures.finement and crowding on HSP70 productionPachygrapsus marmoratus, collected in the Portuguese coast, was investigated–19 mm of carapace width) were confined infinement and crowding during the experimentfication of HSP70 production in the hemolymph, in the three trials. It was concludedficant effect on HSP70 production, periodic handling does not, nor doesfinement to the experimental container or crowding. It can thus be concluded that experimental conditions P. marmoratus and crowding.can thus be used as a model species in climatewarming experiments involving handling, confinement