TY - JOUR
T1 - Effect of Graphene vs. Reduced Graphene Oxide in Gold Nanoparticles for Optical Biosensors
T2 - A Comparative Study
AU - Carvalho, Ana P. G.
AU - Alegria, Elisabete C. B. A.
AU - Fantoni, Alessandro
AU - Ferraria, Ana M.
AU - Do Rego, Ana M. Botelho
AU - Ribeiro, Ana P. C.
N1 - Funding Information:
info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FNAN-OPT%2F31311%2F2017/PT#
info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FQUI-QIN%2F29778%2F2017/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00100%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04565%2F2020/PT#
This research was funded by EU funds through the FEDER European Regional Development Fund (project LISBOA-02-0145-FEDER-031311) project LA/P/0056/2020 of Institute of Molecular Sciences, and LA/P/0140/2020 of i4HB, project UID/EEA/00066/2020 from the Center of Technology and Systems, and from the Instituto Politécnico de Lisboa with IPL/2018/STREAM_ISEL and IPL/2020/AGE-SPReS_ISEL projects. APCR and AMF thank the Instituto Superior Técnico for the scientific employment contracts IST-ID/119/2018 and IST-ID/131/2018, respectively.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/3/4
Y1 - 2022/3/4
N2 - Aiming to develop a nanoparticle-based optical biosensor using gold nanoparticles (AuNPs) synthesized using green methods and supported by carbon-based nanomaterials, we studied the role of carbon derivatives in promoting AuNPs localized surface plasmon resonance (LSPR), as well as their morphology, dispersion, and stability. Carbon derivatives are expected to work as immobi-lization platforms for AuNPs, improving their analytical performance. Gold nanoparticles (AuNPs) were prepared using an eco-friendly approach in a single step by reduction of HAuCl4·3H2O using phytochemicals (from tea) which act as both reducing and capping agents. UV–Vis spectroscopy, transmission electron microscopy (TEM), zeta potential (ζ-potential), and X-ray photoelectron spectroscopy (XPS) were used to characterize the AuNPs and nanocomposites. The addition of reduced graphene oxide (rGO) resulted in greater dispersion of AuNPs on the rGO surface compared with carbon-based nanomaterials used as a support. Differences in morphology due to the nature of the carbon support were observed and are discussed here. AuNPs/rGO seem to be the most promising candidates for the development of LSPR biosensors among the three composites we studied (AuNPs/G, AuNPs/GO, and AuNPs/rGO). Simulations based on the Mie scattering theory have been used to outline the effect of the phytochemicals on LSPR, showing that when the presence of the residuals is limited to the formation of a thin capping layer, the quality of the plasmonic resonance is not affected. A further discussion of the application framework is presented.
AB - Aiming to develop a nanoparticle-based optical biosensor using gold nanoparticles (AuNPs) synthesized using green methods and supported by carbon-based nanomaterials, we studied the role of carbon derivatives in promoting AuNPs localized surface plasmon resonance (LSPR), as well as their morphology, dispersion, and stability. Carbon derivatives are expected to work as immobi-lization platforms for AuNPs, improving their analytical performance. Gold nanoparticles (AuNPs) were prepared using an eco-friendly approach in a single step by reduction of HAuCl4·3H2O using phytochemicals (from tea) which act as both reducing and capping agents. UV–Vis spectroscopy, transmission electron microscopy (TEM), zeta potential (ζ-potential), and X-ray photoelectron spectroscopy (XPS) were used to characterize the AuNPs and nanocomposites. The addition of reduced graphene oxide (rGO) resulted in greater dispersion of AuNPs on the rGO surface compared with carbon-based nanomaterials used as a support. Differences in morphology due to the nature of the carbon support were observed and are discussed here. AuNPs/rGO seem to be the most promising candidates for the development of LSPR biosensors among the three composites we studied (AuNPs/G, AuNPs/GO, and AuNPs/rGO). Simulations based on the Mie scattering theory have been used to outline the effect of the phytochemicals on LSPR, showing that when the presence of the residuals is limited to the formation of a thin capping layer, the quality of the plasmonic resonance is not affected. A further discussion of the application framework is presented.
KW - AuNPs
KW - Biosensors
KW - Metal–graphene hybrid
KW - Mie theory
KW - Simulations
UR - http://www.scopus.com/inward/record.url?scp=85126644894&partnerID=8YFLogxK
U2 - 10.3390/bios12030163
DO - 10.3390/bios12030163
M3 - Article
C2 - 35323433
AN - SCOPUS:85126644894
SN - 2079-6374
VL - 12
JO - Biosensors
JF - Biosensors
IS - 3
M1 - 163
ER -