TY - GEN
T1 - Effect of Epigallocatechin-3-gallate on DMPC Oxidation Revealed by Infrared Spectroscopy
AU - Pires, Filipa
AU - Rodrigues, Bárbara
AU - Magalhães-Mota, Gonçalo
AU - Ribeiro, Paulo A.
AU - Raposo, Maria
N1 - info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FFIS%2FUI0068%2F2011/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FFIS%2F00068%2F2013/PT#
info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FFIS-NAN%2F0909%2F2014/PT#
info:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F106036%2F2015/PT#
© 2020 Springer Nature Switzerland AG
PY - 2020
Y1 - 2020
N2 - The daily exposure of skin cells to the sun increases the rate of production of free radicals, which threatens the healthy appearance of skin and, even more worrying, damages the structural integrity of tissues and DNA, causing inflammation and carcinogenesis. This work demonstrates the feasibility of using natural agents, in particular tea catechins, in protecting lipidic membranes from oxidative stress-induced by UV radiation exposure. For that purpose, thin cast films prepared from vesicular suspensions of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylcholine + (-)-epigallocatechin-3-gallate (DMPC + EGCG) were deposited onto calcium fluoride supports and irradiated with 254 nm UV radiation. The molecular damage after irradiation with UV light was analysed by infrared (IR) together with 2D correlation spectroscopies. Results revealed that the DMPC phospholipid polar moiety is the most vulnerable and sensitive structural target of UV radiation. To check if the presence of the EGCG molecules is protecting the lipids, the principal components analysis (PCA) mathematical method was applied, allowing to conclude that EGCG slows down the cascade of the oxidant-events in the lipid, thus protecting the polar moiety of the lipid.
AB - The daily exposure of skin cells to the sun increases the rate of production of free radicals, which threatens the healthy appearance of skin and, even more worrying, damages the structural integrity of tissues and DNA, causing inflammation and carcinogenesis. This work demonstrates the feasibility of using natural agents, in particular tea catechins, in protecting lipidic membranes from oxidative stress-induced by UV radiation exposure. For that purpose, thin cast films prepared from vesicular suspensions of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylcholine + (-)-epigallocatechin-3-gallate (DMPC + EGCG) were deposited onto calcium fluoride supports and irradiated with 254 nm UV radiation. The molecular damage after irradiation with UV light was analysed by infrared (IR) together with 2D correlation spectroscopies. Results revealed that the DMPC phospholipid polar moiety is the most vulnerable and sensitive structural target of UV radiation. To check if the presence of the EGCG molecules is protecting the lipids, the principal components analysis (PCA) mathematical method was applied, allowing to conclude that EGCG slows down the cascade of the oxidant-events in the lipid, thus protecting the polar moiety of the lipid.
KW - EGCG
KW - Infrared spectroscopy
KW - Lipid oxidation
UR - http://www.scopus.com/inward/record.url?scp=85082132731&partnerID=8YFLogxK
U2 - 10.1007/978-3-030-34585-3_26
DO - 10.1007/978-3-030-34585-3_26
M3 - Conference contribution
AN - SCOPUS:85082132731
SN - 978-3-030-34584-6
T3 - Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
SP - 295
EP - 302
BT - Computational Intelligence Methods for Bioinformatics and Biostatistics - 15th International Meeting, CIBB 2018, Revised Selected Papers
A2 - Raposo, Maria
A2 - Ribeiro, Paulo
A2 - Sério, Susana
A2 - Staiano, Antonino
A2 - Ciaramella, Angelo
PB - Springer
CY - Cham
T2 - 15th International Conference on Computational Intelligence Methods for Bioinformatics and Biostatistics, CIBB 2018
Y2 - 6 September 2018 through 8 September 2018
ER -