TY - JOUR
T1 - Effect of carbonic anhydrase on CO2 absorption promoted by choline hydroxide using supported liquid membranes
AU - Castro, Aline Machado de
AU - Neves, Luísa A.
AU - Corvo, Marta C.
AU - Cabrita, Eurico J.
AU - Crespo, João G.
N1 - Funding Information:
AMC acknowledges the research funds from PETROBRAS and The National Council for Scientific and Technological Development ( CNPq , Brazil, process 201500/2018-3 ). This work was also supported by the Associate Laboratory for Green Chemistry – LAQV ( UIDB/50006/2020 and UIDP/50006/2020 ) and the Applied Molecular Biosciences Unit - UCIBIO (UIDB/04378/2020), which are financed by national funds from FCT/MCTES and Fundação para a Ciência e a Tecnologia and the exploratory project (IF/00505/2014/CP1224/CT0004) attributed to LAN within the 2014 FCT Investigator Programme. The NMR spectrometers at FCT NOVA are part of Rede Nacional de RMN (PTNMR), supported by FCT- Fundação para a Ciência e a Tecnologia (ROTEIRO/0031/2013 - PINFRA/22161/2016) co-financed by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC). MCC gratefully acknowledges PTNMR for the researcher contract and UIDB/50025/2020-2023. Prof. Luisa Ferreira, Ms. Elisabete Ferreira and Ms. Paloma Albo are acknowledged for their support in FT-IR, nano-DSC analysis and data modeling, respectively. We are also grateful to Dr. Mohammad Tariq and Dr. José Esperança for their assistance in density and viscosity analysis.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - The development of efficient CO2 capture routes to tackle the unprecedently high concentrations of this gas in the atmosphere is one of the world's top-priority technological targets. Here, we describe, for the first time, the combined use of the enzyme carbonic anhydrase (CA) and an aqueous solution of choline hydroxide, to effectively absorb CO2. Variables such as choline hydroxide and enzyme concentrations and temperature were investigated. Overall sorption coefficients of up to 105 gCO2 kg−1solution were found (up to 3.8 M absorbent), whereas reaction rate improvements were demonstrated within a low enzyme concentration range (0.1–0.5 mg g−1). FT-IR and in situ 1H and 13C NMR experiments supported the elucidation of reaction mechanisms. It was found that in the absence of CA, the choline carbonate species is rapidly shifted to the bicarbonate form, whereas in the presence of the enzyme, the carbonate form is stable, conferring a buffered-like environment. Finally, the solvents containing the enzyme were used for the preparation of supported liquid membranes, which showed CO2 selectivity to N2 and CH4 of up to 90.5 and 28.9 times, respectively. The results here discussed add relevant knowledge towards the implementation of efficient CO2 capture.
AB - The development of efficient CO2 capture routes to tackle the unprecedently high concentrations of this gas in the atmosphere is one of the world's top-priority technological targets. Here, we describe, for the first time, the combined use of the enzyme carbonic anhydrase (CA) and an aqueous solution of choline hydroxide, to effectively absorb CO2. Variables such as choline hydroxide and enzyme concentrations and temperature were investigated. Overall sorption coefficients of up to 105 gCO2 kg−1solution were found (up to 3.8 M absorbent), whereas reaction rate improvements were demonstrated within a low enzyme concentration range (0.1–0.5 mg g−1). FT-IR and in situ 1H and 13C NMR experiments supported the elucidation of reaction mechanisms. It was found that in the absence of CA, the choline carbonate species is rapidly shifted to the bicarbonate form, whereas in the presence of the enzyme, the carbonate form is stable, conferring a buffered-like environment. Finally, the solvents containing the enzyme were used for the preparation of supported liquid membranes, which showed CO2 selectivity to N2 and CH4 of up to 90.5 and 28.9 times, respectively. The results here discussed add relevant knowledge towards the implementation of efficient CO2 capture.
KW - Carbon dioxide
KW - Carbonic anhydrase enzyme
KW - Choline hydroxide
KW - CO capture
KW - Supported liquid membrane
UR - http://www.scopus.com/inward/record.url?scp=85117267242&partnerID=8YFLogxK
U2 - 10.1016/j.seppur.2021.119921
DO - 10.1016/j.seppur.2021.119921
M3 - Article
AN - SCOPUS:85117267242
SN - 1383-5866
VL - 280
JO - Separation and Purification Technology
JF - Separation and Purification Technology
M1 - 119921
ER -