Effect of blue light irradiation on the stability of phospholipid molecules in the presence of epigallocatechin-3-gallate

Filipa Pires, Vananelia P. N. Geraldo, Andrea Antunes, Alexandre Marletta, Osvaldo N. Oliveira, Maria Raposo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this paper, we report on the effects from epigallocatechin-3-gallate (EGCG), a phytochemical flavonoid present in green tea, on Langmuir monolayers of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol) (sodium salt) (DPPG), including experiments with blue light irradiation. EGCG was found to interact with both the DPPG headgroups and hydrophobic tails, thus affecting the lipid packing according to surface pressure and surface potential isotherms and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) data. Blue light irradiation caused considerable changes in the surface pressure isotherms and PM-IRRAS spectra of DPPG monolayers, but the effects were considerably less when EGCG was present. For the surface pressure isotherms, for instance, no irradiation effect could be measured for mixed EGCG-DPPG monolayers. It is concluded that EGCG protected the DPPG molecules from degrading upon blue light irradiation, which means that EGCG may be a preventive and therapeutic agent to decrease photosensitivity of phospholipids to blue light oxidative damage, a pathogenic mechanism in skin disorders.

Original languageEnglish
Pages (from-to)50-57
Number of pages8
JournalColloids And Surfaces B-Biointerfaces
Volume177
DOIs
Publication statusE-pub ahead of print - 23 Jan 2019

Fingerprint

gallates
Phospholipids
Irradiation
Isotherms
Light
Monolayers
Molecules
irradiation
Absorption spectroscopy
molecules
isotherms
infrared reflection
Polarization
Infrared radiation
Pressure
Flavonoids
Photosensitivity
Spectrum Analysis
absorption spectroscopy
Surface potential

Keywords

  • Blue light radiation
  • Catechins
  • DPPG
  • Drug delivery systems
  • EGCG
  • IRRAS
  • Langmuir
  • Liposome

Cite this

Pires, Filipa ; Geraldo, Vananelia P. N. ; Antunes, Andrea ; Marletta, Alexandre ; Oliveira, Osvaldo N. ; Raposo, Maria. / Effect of blue light irradiation on the stability of phospholipid molecules in the presence of epigallocatechin-3-gallate. In: Colloids And Surfaces B-Biointerfaces. 2019 ; Vol. 177. pp. 50-57.
@article{a2a7c02b58374efc85133ae6069892e4,
title = "Effect of blue light irradiation on the stability of phospholipid molecules in the presence of epigallocatechin-3-gallate",
abstract = "In this paper, we report on the effects from epigallocatechin-3-gallate (EGCG), a phytochemical flavonoid present in green tea, on Langmuir monolayers of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol) (sodium salt) (DPPG), including experiments with blue light irradiation. EGCG was found to interact with both the DPPG headgroups and hydrophobic tails, thus affecting the lipid packing according to surface pressure and surface potential isotherms and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) data. Blue light irradiation caused considerable changes in the surface pressure isotherms and PM-IRRAS spectra of DPPG monolayers, but the effects were considerably less when EGCG was present. For the surface pressure isotherms, for instance, no irradiation effect could be measured for mixed EGCG-DPPG monolayers. It is concluded that EGCG protected the DPPG molecules from degrading upon blue light irradiation, which means that EGCG may be a preventive and therapeutic agent to decrease photosensitivity of phospholipids to blue light oxidative damage, a pathogenic mechanism in skin disorders.",
keywords = "Blue light radiation, Catechins, DPPG, Drug delivery systems, EGCG, IRRAS, Langmuir, Liposome",
author = "Filipa Pires and Geraldo, {Vananelia P. N.} and Andrea Antunes and Alexandre Marletta and Oliveira, {Osvaldo N.} and Maria Raposo",
note = "info:eu-repo/grantAgreement/FCT/5876/147412/PT# PTDC/FIS-NAN/0909/2014. PEst-OE/FIS/UI0068/2011. FAPESP (2013/14262-7). PD/BD/106036/2015. Sem PDF conforme despacho.",
year = "2019",
month = "1",
day = "23",
doi = "10.1016/j.colsurfb.2019.01.042",
language = "English",
volume = "177",
pages = "50--57",
journal = "Colloids And Surfaces B-Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier Science B.V., Amsterdam.",

}

Effect of blue light irradiation on the stability of phospholipid molecules in the presence of epigallocatechin-3-gallate. / Pires, Filipa; Geraldo, Vananelia P. N.; Antunes, Andrea; Marletta, Alexandre; Oliveira, Osvaldo N.; Raposo, Maria.

In: Colloids And Surfaces B-Biointerfaces, Vol. 177, 23.01.2019, p. 50-57.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of blue light irradiation on the stability of phospholipid molecules in the presence of epigallocatechin-3-gallate

AU - Pires, Filipa

AU - Geraldo, Vananelia P. N.

AU - Antunes, Andrea

AU - Marletta, Alexandre

AU - Oliveira, Osvaldo N.

AU - Raposo, Maria

N1 - info:eu-repo/grantAgreement/FCT/5876/147412/PT# PTDC/FIS-NAN/0909/2014. PEst-OE/FIS/UI0068/2011. FAPESP (2013/14262-7). PD/BD/106036/2015. Sem PDF conforme despacho.

PY - 2019/1/23

Y1 - 2019/1/23

N2 - In this paper, we report on the effects from epigallocatechin-3-gallate (EGCG), a phytochemical flavonoid present in green tea, on Langmuir monolayers of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol) (sodium salt) (DPPG), including experiments with blue light irradiation. EGCG was found to interact with both the DPPG headgroups and hydrophobic tails, thus affecting the lipid packing according to surface pressure and surface potential isotherms and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) data. Blue light irradiation caused considerable changes in the surface pressure isotherms and PM-IRRAS spectra of DPPG monolayers, but the effects were considerably less when EGCG was present. For the surface pressure isotherms, for instance, no irradiation effect could be measured for mixed EGCG-DPPG monolayers. It is concluded that EGCG protected the DPPG molecules from degrading upon blue light irradiation, which means that EGCG may be a preventive and therapeutic agent to decrease photosensitivity of phospholipids to blue light oxidative damage, a pathogenic mechanism in skin disorders.

AB - In this paper, we report on the effects from epigallocatechin-3-gallate (EGCG), a phytochemical flavonoid present in green tea, on Langmuir monolayers of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol) (sodium salt) (DPPG), including experiments with blue light irradiation. EGCG was found to interact with both the DPPG headgroups and hydrophobic tails, thus affecting the lipid packing according to surface pressure and surface potential isotherms and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) data. Blue light irradiation caused considerable changes in the surface pressure isotherms and PM-IRRAS spectra of DPPG monolayers, but the effects were considerably less when EGCG was present. For the surface pressure isotherms, for instance, no irradiation effect could be measured for mixed EGCG-DPPG monolayers. It is concluded that EGCG protected the DPPG molecules from degrading upon blue light irradiation, which means that EGCG may be a preventive and therapeutic agent to decrease photosensitivity of phospholipids to blue light oxidative damage, a pathogenic mechanism in skin disorders.

KW - Blue light radiation

KW - Catechins

KW - DPPG

KW - Drug delivery systems

KW - EGCG

KW - IRRAS

KW - Langmuir

KW - Liposome

UR - http://www.scopus.com/inward/record.url?scp=85060638989&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2019.01.042

DO - 10.1016/j.colsurfb.2019.01.042

M3 - Article

VL - 177

SP - 50

EP - 57

JO - Colloids And Surfaces B-Biointerfaces

JF - Colloids And Surfaces B-Biointerfaces

SN - 0927-7765

ER -