Effect of a poly(ADP-ribose) polymerase inhibitor on DNA breakage and cytotoxicity induced by hydrogen peroxide and γ-radiation

Luísa Cristóvão, José Rueff

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The activity of poly(ADP-ribose) polymerase (PARP) is activated upon recognition of DNA strand breaks by its DNA-binding domain and the stimulation of this nuclear enzyme seems to be an early response of cells exposed to a variety of different DNA-damaging agents. In the present work we evaluate the effect of hydrogen peroxide and γ-radiation on DNA strand breaks in human leukocytes in the presence and in the absence of 3-aminobenzamide (3AB), a potent inhibitor of PARP. Our results have shown differences in the role poly(ADP-ribosyl)ation in the rejoining of DNA strand breaks induced by hydrogen peroxide and γ-radiation. We observed a PARP-dependent recovery of DNA strand breaks at the incubation time of 20-30 min in leukocytes treated with hydrogen peroxide. The repair of DNA strand breaks induced by γ-radiation seems to be dependent on oxygen radical scavenging and the stimulation of PARP could be related to the protection of DNA strand breaks from hydroxyl radicals.

Original languageEnglish
Pages (from-to)219-227
Number of pages9
JournalTeratogenesis Carcinogenesis And Mutagenesis
Volume16
Issue number4
DOIs
Publication statusPublished - 1 Dec 1996

Keywords

  • γ-radiation
  • DNA repair
  • DNA strand breaks
  • Hydroxyl radical
  • Oxidative DNA damage
  • Oxygen species
  • Poly(ADP-ribose) polymerase

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