Ecophysiological responses of juvenile seabass (Dicentrarchus labrax) exposed to increased temperature and dietary methylmercury

Ana Luísa Maulvault, Vera Barbosa, Ricardo Pinheiro-Alves, Ana Custódio, Patrícia Anacleto, Tiago Repolho, Pedro Pousão-Ferreira, Rui Rosa, António Marques, Mário Diniz

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18 Citations (Scopus)

Abstract

The ecotoxicological effects of methylmercury (MeHg) exposure have been intensively described in literature. Yet, it is still unclear how marine biota will respond to the presence of MeHg under climate change, namely ocean warming. The present study aimed to investigate, for the first time, fish condition [Fulton's K index (K), hepatosomatic index (HIS) and brain-to-body mass ratio (BB-ratio)] and several stress-related responses in an ecologically and commercially important fish species (Dicentrachus labrax) exposed for 28 days to dietary MeHg (8.0 mg kg-1 dw) and temperature increase (+ 4 °C). Results showed significant impairments on fish condition, i.e. up to 34% decrease on K, > 100% increase on HIS and 44% decrease on BB-ratio, compared to control conditions. Significant changes on tissue biochemical responses were observed in fish exposed to both stressors, acting alone or combined, evidencing the relevance of assessing possible interactions between different environmental stressors in ecotoxicological studies. For instance, muscle showed to be the least affected tissue, only revealing significant alterations in GST activity of MeHg-enriched fish. On the other hand, liver exhibited a significant induction of GST (> 100%) and CAT (up to 74%) in MeHg-enriched fish, regardless of temperature exposure, as well as decreased SOD activity (19%) and increased HSP70/HSC70 content (87%) in fish exposed to warming alone. Brain showed to be affected by temperature (69% of GST inhibition and > 100% of increased CAT activity), MeHg (> 100% of increased CAT activity, 47% of SOD inhibition and 55% of AChE inhibition), as well as by the combination of both (GST, SOD and AChE inhibition, 17%, 48% and 53%, respectively). Hence, our data provides evidences that the toxicological aspects of MeHg ca be potentiated by warmer temperatures, thus, evidencing the need for further research combining contaminants exposure and climate change effects, to better forecast ecological impacts in the ocean of tomorrow.

Original languageEnglish
Pages (from-to)551-558
Number of pages8
JournalScience of the Total Environment
Volume586
DOIs
Publication statusPublished - 15 May 2017

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methylmercury
Fish
fish
temperature
brain
Brain
Temperature
Climate change
body mass
warming
Tissue
climate change
ocean
ecological impact
Liver
Muscle
biota
muscle
Impurities
pollutant

Keywords

  • Animal condition
  • Dietary MeHg
  • Heat shock
  • Neurotoxicity
  • Oxidative stress
  • Seawater warming

Cite this

Maulvault, Ana Luísa ; Barbosa, Vera ; Pinheiro-Alves, Ricardo ; Custódio, Ana ; Anacleto, Patrícia ; Repolho, Tiago ; Pousão-Ferreira, Pedro ; Rosa, Rui ; Marques, António ; Diniz, Mário. / Ecophysiological responses of juvenile seabass (Dicentrarchus labrax) exposed to increased temperature and dietary methylmercury. In: Science of the Total Environment. 2017 ; Vol. 586. pp. 551-558.
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abstract = "The ecotoxicological effects of methylmercury (MeHg) exposure have been intensively described in literature. Yet, it is still unclear how marine biota will respond to the presence of MeHg under climate change, namely ocean warming. The present study aimed to investigate, for the first time, fish condition [Fulton's K index (K), hepatosomatic index (HIS) and brain-to-body mass ratio (BB-ratio)] and several stress-related responses in an ecologically and commercially important fish species (Dicentrachus labrax) exposed for 28 days to dietary MeHg (8.0 mg kg-1 dw) and temperature increase (+ 4 °C). Results showed significant impairments on fish condition, i.e. up to 34{\%} decrease on K, > 100{\%} increase on HIS and 44{\%} decrease on BB-ratio, compared to control conditions. Significant changes on tissue biochemical responses were observed in fish exposed to both stressors, acting alone or combined, evidencing the relevance of assessing possible interactions between different environmental stressors in ecotoxicological studies. For instance, muscle showed to be the least affected tissue, only revealing significant alterations in GST activity of MeHg-enriched fish. On the other hand, liver exhibited a significant induction of GST (> 100{\%}) and CAT (up to 74{\%}) in MeHg-enriched fish, regardless of temperature exposure, as well as decreased SOD activity (19{\%}) and increased HSP70/HSC70 content (87{\%}) in fish exposed to warming alone. Brain showed to be affected by temperature (69{\%} of GST inhibition and > 100{\%} of increased CAT activity), MeHg (> 100{\%} of increased CAT activity, 47{\%} of SOD inhibition and 55{\%} of AChE inhibition), as well as by the combination of both (GST, SOD and AChE inhibition, 17{\%}, 48{\%} and 53{\%}, respectively). Hence, our data provides evidences that the toxicological aspects of MeHg ca be potentiated by warmer temperatures, thus, evidencing the need for further research combining contaminants exposure and climate change effects, to better forecast ecological impacts in the ocean of tomorrow.",
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Maulvault, AL, Barbosa, V, Pinheiro-Alves, R, Custódio, A, Anacleto, P, Repolho, T, Pousão-Ferreira, P, Rosa, R, Marques, A & Diniz, M 2017, 'Ecophysiological responses of juvenile seabass (Dicentrarchus labrax) exposed to increased temperature and dietary methylmercury', Science of the Total Environment, vol. 586, pp. 551-558. https://doi.org/10.1016/j.scitotenv.2017.02.016

Ecophysiological responses of juvenile seabass (Dicentrarchus labrax) exposed to increased temperature and dietary methylmercury. / Maulvault, Ana Luísa; Barbosa, Vera; Pinheiro-Alves, Ricardo; Custódio, Ana; Anacleto, Patrícia; Repolho, Tiago; Pousão-Ferreira, Pedro; Rosa, Rui; Marques, António; Diniz, Mário.

In: Science of the Total Environment, Vol. 586, 15.05.2017, p. 551-558.

Research output: Contribution to journalArticle

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T1 - Ecophysiological responses of juvenile seabass (Dicentrarchus labrax) exposed to increased temperature and dietary methylmercury

AU - Maulvault, Ana Luísa

AU - Barbosa, Vera

AU - Pinheiro-Alves, Ricardo

AU - Custódio, Ana

AU - Anacleto, Patrícia

AU - Repolho, Tiago

AU - Pousão-Ferreira, Pedro

AU - Rosa, Rui

AU - Marques, António

AU - Diniz, Mário

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N2 - The ecotoxicological effects of methylmercury (MeHg) exposure have been intensively described in literature. Yet, it is still unclear how marine biota will respond to the presence of MeHg under climate change, namely ocean warming. The present study aimed to investigate, for the first time, fish condition [Fulton's K index (K), hepatosomatic index (HIS) and brain-to-body mass ratio (BB-ratio)] and several stress-related responses in an ecologically and commercially important fish species (Dicentrachus labrax) exposed for 28 days to dietary MeHg (8.0 mg kg-1 dw) and temperature increase (+ 4 °C). Results showed significant impairments on fish condition, i.e. up to 34% decrease on K, > 100% increase on HIS and 44% decrease on BB-ratio, compared to control conditions. Significant changes on tissue biochemical responses were observed in fish exposed to both stressors, acting alone or combined, evidencing the relevance of assessing possible interactions between different environmental stressors in ecotoxicological studies. For instance, muscle showed to be the least affected tissue, only revealing significant alterations in GST activity of MeHg-enriched fish. On the other hand, liver exhibited a significant induction of GST (> 100%) and CAT (up to 74%) in MeHg-enriched fish, regardless of temperature exposure, as well as decreased SOD activity (19%) and increased HSP70/HSC70 content (87%) in fish exposed to warming alone. Brain showed to be affected by temperature (69% of GST inhibition and > 100% of increased CAT activity), MeHg (> 100% of increased CAT activity, 47% of SOD inhibition and 55% of AChE inhibition), as well as by the combination of both (GST, SOD and AChE inhibition, 17%, 48% and 53%, respectively). Hence, our data provides evidences that the toxicological aspects of MeHg ca be potentiated by warmer temperatures, thus, evidencing the need for further research combining contaminants exposure and climate change effects, to better forecast ecological impacts in the ocean of tomorrow.

AB - The ecotoxicological effects of methylmercury (MeHg) exposure have been intensively described in literature. Yet, it is still unclear how marine biota will respond to the presence of MeHg under climate change, namely ocean warming. The present study aimed to investigate, for the first time, fish condition [Fulton's K index (K), hepatosomatic index (HIS) and brain-to-body mass ratio (BB-ratio)] and several stress-related responses in an ecologically and commercially important fish species (Dicentrachus labrax) exposed for 28 days to dietary MeHg (8.0 mg kg-1 dw) and temperature increase (+ 4 °C). Results showed significant impairments on fish condition, i.e. up to 34% decrease on K, > 100% increase on HIS and 44% decrease on BB-ratio, compared to control conditions. Significant changes on tissue biochemical responses were observed in fish exposed to both stressors, acting alone or combined, evidencing the relevance of assessing possible interactions between different environmental stressors in ecotoxicological studies. For instance, muscle showed to be the least affected tissue, only revealing significant alterations in GST activity of MeHg-enriched fish. On the other hand, liver exhibited a significant induction of GST (> 100%) and CAT (up to 74%) in MeHg-enriched fish, regardless of temperature exposure, as well as decreased SOD activity (19%) and increased HSP70/HSC70 content (87%) in fish exposed to warming alone. Brain showed to be affected by temperature (69% of GST inhibition and > 100% of increased CAT activity), MeHg (> 100% of increased CAT activity, 47% of SOD inhibition and 55% of AChE inhibition), as well as by the combination of both (GST, SOD and AChE inhibition, 17%, 48% and 53%, respectively). Hence, our data provides evidences that the toxicological aspects of MeHg ca be potentiated by warmer temperatures, thus, evidencing the need for further research combining contaminants exposure and climate change effects, to better forecast ecological impacts in the ocean of tomorrow.

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