TY - JOUR
T1 - Early detection of grapevine graft incompatibility
T2 - Insights into translocated and virus-induced incompatibility
AU - Tedesco, Sara
AU - Irisarri, Patricia
AU - Teixeira Santos, Margarida
AU - Fevereiro, Pedro
AU - Pina, Ana
AU - Kragler, Friedrich
N1 - Funding Information:
This research was supported by internal funds provided to FK from the Max Planck Society, Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021. The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón- European Social Fund, European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively.
Funding Information:
This research was supported by internal funds provided to FK from the Max Planck Society , Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021 . The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón - European Social Fund , European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively.
Publisher Copyright:
© 2023
PY - 2023/8/1
Y1 - 2023/8/1
N2 - In vineyards to control phylloxera (Daktulosphaira vitifolia Ficth) attacks in Vitis vinifera L., heterografted vines are planted using American vines hybrids as rootstocks. However, graft incompatibilities can affect grape yield and plant longevity. Thus, to identify early graft incompatibility factors, we established in vitro micrografting protocols coupled with histology and histochemistry analysis in grapevine graft combinations of known compatibility behavior. The histochemical characterization of the graft union revealed irregular cell arrangement, slower vascular differentiation, persistence of the necrotic layer, accumulation of starch, and lower differentiation of phloem cells in hetero- compared to homografts, indicating the presence of translocated incompatibility symptoms. We highlight the utility of evaluating the graft interface cellular arrangement and starch content via calcofluor and I2KI staining, respectively, as allowed to identify the graft combinations with lower graft success. Wounded and grafted Syrah plantlets pointed out an impaired sucrose distribution in these plants and levels of Grapevine Rupestris Stem Pitting associated Virus (GRSPaV) infections correlated with graft (un)-success in two Syrah clones micrografted onto 110-Ritcher rootstock. Furthermore, silencing GRSPaV before grafting increased graft success rates. We propose that grapevine graft incompatibility is mainly a virus-induced phenomenon that can arise even in certified plants.
AB - In vineyards to control phylloxera (Daktulosphaira vitifolia Ficth) attacks in Vitis vinifera L., heterografted vines are planted using American vines hybrids as rootstocks. However, graft incompatibilities can affect grape yield and plant longevity. Thus, to identify early graft incompatibility factors, we established in vitro micrografting protocols coupled with histology and histochemistry analysis in grapevine graft combinations of known compatibility behavior. The histochemical characterization of the graft union revealed irregular cell arrangement, slower vascular differentiation, persistence of the necrotic layer, accumulation of starch, and lower differentiation of phloem cells in hetero- compared to homografts, indicating the presence of translocated incompatibility symptoms. We highlight the utility of evaluating the graft interface cellular arrangement and starch content via calcofluor and I2KI staining, respectively, as allowed to identify the graft combinations with lower graft success. Wounded and grafted Syrah plantlets pointed out an impaired sucrose distribution in these plants and levels of Grapevine Rupestris Stem Pitting associated Virus (GRSPaV) infections correlated with graft (un)-success in two Syrah clones micrografted onto 110-Ritcher rootstock. Furthermore, silencing GRSPaV before grafting increased graft success rates. We propose that grapevine graft incompatibility is mainly a virus-induced phenomenon that can arise even in certified plants.
KW - Graft incompatibility
KW - Grapevine
KW - GRSPaV
KW - Histology
KW - In vitro culture
KW - Syrah
UR - https://www.scopus.com/pages/publications/85153623372
U2 - 10.1016/j.scienta.2023.112087
DO - 10.1016/j.scienta.2023.112087
M3 - Article
AN - SCOPUS:85153623372
SN - 0304-4238
VL - 318
JO - Scientia Horticulturae
JF - Scientia Horticulturae
M1 - 112087
ER -