Earliest events in α-synuclein fibrillation probed with the fluorescence of intrinsic tyrosines

Marco A. Saraiva, Carla Alexandra Jorge, Maria Helena Santos, António L. Maçanita

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The fluorescence of the four tyrosines of α-synuclein (Syn) was used for probing the earliest events preceding the fibrillation of Syn, during the onset of the so-called lag-time of fibrillation. Steady-state fluorescence experiments revealed an increase in the fluorescence intensity (FI) for Syn solutions at pH values 3 and 2, in comparison with pH 7, and fluorescence decays indicated that the FI increase did not result from suppression of excited-state proton transfer from the tyrosines to aspartates and glutamates, exposure of tyrosines to more hydrophobic environments, or reduction of homo-energy transfer. Instead, the FI increase was due to changes in the population of the tyrosine rotamers at low pH values. Stopped-flow experiments (pH-jumps) showed that the FI enhancement involves two processes: a fast (sub-7 ms) intramolecular (concentration-independent) process, which we assign to the protein collapse at low pH, and a slower intermolecular (concentration-dependent) process of protein dimerization/oligomerization, starting at 4-10 s after acidification. To the best of our knowledge, this is the first work on the experimental detection of these earliest processes in the fibrillation of Syn.

Original languageEnglish
Pages (from-to)16-23
Number of pages8
JournalJournal Of Photochemistry And Photobiology B-Biology
Volume154
DOIs
Publication statusPublished - 1 Jan 2016

Keywords

  • Intrinsic fluorescence probe
  • pH-jump
  • Protein aggregation
  • Stopped-flow

Fingerprint Dive into the research topics of 'Earliest events in α-synuclein fibrillation probed with the fluorescence of intrinsic tyrosines'. Together they form a unique fingerprint.

Cite this