Abstract

Spatial and temporal control of molecular mechanisms can be achieved using photolabile bonds that connect biomolecules to protective caging groups, which can be cleaved upon irradiation of a specific wavelength, releasing the biomolecule ready-to-use. Here we apply and improve a previously reported strategy to tightly control in vitro transcription reactions. The strategy involves two caging molecules that block both ATP and GTP nucleotides. Additionally, we designed a molecular beacon complementary to the synthesized mRNA to infer its presence through a light signal. Upon release of both nucleotides through a specific monochromatic light (390 and 325 nm) we attain a light signal indicative of a successful in vitro transcription reaction. Similarly, in the absence of irradiation, no intense fluorescence signal was obtained. We believe this strategy could further be applied to DNA synthesis or the development of logic gates.

Original languageEnglish
Pages (from-to)751-756
Number of pages6
JournalPhotochemical and Photobiological Sciences
Volume13
Issue number5
DOIs
Publication statusPublished - 2014

Keywords

  • MOLECULAR BEACONS
  • PHOTOCLEAVAGE
  • ESTERS
  • TOOLS
  • RNA

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