DNA strand breaks and chromosomal aberrations induced by H2O2 and 60Co gamma-radiation

J. Rueff; A. Brás; L Cristóvão; J Mexia; M Sá da Costa; V Pires

DNA strand breaks and chromosomal aberrations induced by H2O2 and 60Co gamma-radiation

J. Rueff, A. Brás, L Cristóvão, J Mexia, M Sá da Costa, V Pires

NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)

Research output: Contribution to journal › Article

59 Citations (Scopus)

Abstract

DNA strand breaks and chromosomal aberrations (CAs) were studied in human cells treated with hydrogen peroxide or with ionizing radiation. DNA strand breaks could be produced at dose levels of H2O2 much lower than those which induced CAs. Doses as low as 0.5 mM of H2O2 produced about as many DNA strand breaks as 2 Gy of 60Co gamma-radiation. On the other hand, as much as 20 mM H2O2 produced only half as many CAs as 1 Gy of 60Co gamma-radiation. The different mechanisms involved in the production of human genetic damage by H2O2 and gamma-radiation are discussed.

Original language	English
Pages (from-to)	197-204
Number of pages	8
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	289
Issue number	2
Publication status	Published - Oct 1993

Keywords

Adult
Cells, Cultured
Chromosome Aberrations
Cobalt Radioisotopes
DNA
DNA Damage
Gamma Rays
Humans
Hydrogen Peroxide
Leukocytes
Middle Aged
Journal Article
Research Support, Non-U.S. Gov't

Fingerprint Dive into the research topics of 'DNA strand breaks and chromosomal aberrations induced by H2O2 and 60Co gamma-radiation'. Together they form a unique fingerprint.

Cite this

@article{f566260f7d0a47c4a06f6226959b4960,

title = "DNA strand breaks and chromosomal aberrations induced by H2O2 and 60Co gamma-radiation",

abstract = "DNA strand breaks and chromosomal aberrations (CAs) were studied in human cells treated with hydrogen peroxide or with ionizing radiation. DNA strand breaks could be produced at dose levels of H2O2 much lower than those which induced CAs. Doses as low as 0.5 mM of H2O2 produced about as many DNA strand breaks as 2 Gy of 60Co gamma-radiation. On the other hand, as much as 20 mM H2O2 produced only half as many CAs as 1 Gy of 60Co gamma-radiation. The different mechanisms involved in the production of human genetic damage by H2O2 and gamma-radiation are discussed.",

keywords = "Adult, Cells, Cultured, Chromosome Aberrations, Cobalt Radioisotopes, DNA, DNA Damage, Gamma Rays, Humans, Hydrogen Peroxide, Leukocytes, Middle Aged, Journal Article, Research Support, Non-U.S. Gov't",

author = "J. Rueff and A. Br{\'a}s and L Crist{\'o}v{\~a}o and J Mexia and {S{\'a} da Costa}, M and V Pires",

note = "PMID:7690888 ",

year = "1993",

month = oct,

language = "English",

volume = "289",

pages = "197--204",

journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",

issn = "0027-5107",

publisher = "Elsevier Science B.V., Inc",

number = "2",

}

TY - JOUR

T1 - DNA strand breaks and chromosomal aberrations induced by H2O2 and 60Co gamma-radiation

AU - Rueff, J.

AU - Brás, A.

AU - Cristóvão, L

AU - Mexia, J

AU - Sá da Costa, M

AU - Pires, V

N1 - PMID:7690888

PY - 1993/10

Y1 - 1993/10

N2 - DNA strand breaks and chromosomal aberrations (CAs) were studied in human cells treated with hydrogen peroxide or with ionizing radiation. DNA strand breaks could be produced at dose levels of H2O2 much lower than those which induced CAs. Doses as low as 0.5 mM of H2O2 produced about as many DNA strand breaks as 2 Gy of 60Co gamma-radiation. On the other hand, as much as 20 mM H2O2 produced only half as many CAs as 1 Gy of 60Co gamma-radiation. The different mechanisms involved in the production of human genetic damage by H2O2 and gamma-radiation are discussed.

AB - DNA strand breaks and chromosomal aberrations (CAs) were studied in human cells treated with hydrogen peroxide or with ionizing radiation. DNA strand breaks could be produced at dose levels of H2O2 much lower than those which induced CAs. Doses as low as 0.5 mM of H2O2 produced about as many DNA strand breaks as 2 Gy of 60Co gamma-radiation. On the other hand, as much as 20 mM H2O2 produced only half as many CAs as 1 Gy of 60Co gamma-radiation. The different mechanisms involved in the production of human genetic damage by H2O2 and gamma-radiation are discussed.

KW - Adult

KW - Cells, Cultured

KW - Chromosome Aberrations

KW - Cobalt Radioisotopes

KW - DNA

KW - DNA Damage

KW - Gamma Rays

KW - Humans

KW - Hydrogen Peroxide

KW - Leukocytes

KW - Middle Aged

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

C2 - 7690888

VL - 289

SP - 197

EP - 204

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

SN - 0027-5107

IS - 2

ER -