TY - JOUR
T1 - Diversity of methicillin-resistant staphylococci among wild Lepus granatensis: First detection of mecA-MRSA in hares
AU - Silva, Vanessa
AU - Pereira, José Eduardo
AU - Maltez, Luís
AU - Ferreira, Eugénia
AU - Manageiro, Vera
AU - Caniça, Manuela
AU - Capelo, José L.
AU - Igrejas, Gilberto
AU - Poeta, Patrícia
N1 - Funding Information:
This work was funded by the R&D Project CAREBIO2?Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics?towards innovative theranostic biomarkers, with reference NORTE-01?0145FEDER-030101 and PTDC/SAU-INF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry-LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019).
Vanessa Silva is supported by national funds through FCT/MCTES and by the European Social Fund through POCH/FSE under the PhD grant SFRH/BD/137 947/2018.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - MRSA in humans, pets and livestock have been widely investigated, nevertheless, there is still little information of MRSA in wild animals. Therefore, this study aimed to investigate the occurrence and antimicrobial resistance profiles of methicillin-resistant staphylococci (MRS) in wild Iberian hares and to characterize their genetic lineages. Samples from 83 wild hares (Lepus granatensis) were collected during the hunting season. Isolation of MRS was accomplish using Oxacillin Resistant Screening Agar medium with 2 mg/L of oxacillin. The susceptibility of the isolates was tested by the Kirby-Bauer disc diffusion method. The presence of resistance and virulence genes was studied by PCR. S. aureus strains were further characterized by multilocus sequence typing, agr, spa and SCCmec typing. From the 83 samples, 12 (14.45%) coagulase-negative staphylococci and 3 (3.6%) MRSA strains were isolated. Nine coagulase-negative isolates showed resistance to at least one antibiotic. One MRSA isolate showed a multidrug-resistant profile with resistances to ß-lactams, aminoglycosides, macrolides and lincosamides. All MRSA strains were ascribed to ST2855, t1190 and SCCmec type III. The frequency of MRSA strains in wild hares was low, nevertheless, the presence of MRSA in game animals is considered a public health problem and may represent a route of transmission between animals and humans.
AB - MRSA in humans, pets and livestock have been widely investigated, nevertheless, there is still little information of MRSA in wild animals. Therefore, this study aimed to investigate the occurrence and antimicrobial resistance profiles of methicillin-resistant staphylococci (MRS) in wild Iberian hares and to characterize their genetic lineages. Samples from 83 wild hares (Lepus granatensis) were collected during the hunting season. Isolation of MRS was accomplish using Oxacillin Resistant Screening Agar medium with 2 mg/L of oxacillin. The susceptibility of the isolates was tested by the Kirby-Bauer disc diffusion method. The presence of resistance and virulence genes was studied by PCR. S. aureus strains were further characterized by multilocus sequence typing, agr, spa and SCCmec typing. From the 83 samples, 12 (14.45%) coagulase-negative staphylococci and 3 (3.6%) MRSA strains were isolated. Nine coagulase-negative isolates showed resistance to at least one antibiotic. One MRSA isolate showed a multidrug-resistant profile with resistances to ß-lactams, aminoglycosides, macrolides and lincosamides. All MRSA strains were ascribed to ST2855, t1190 and SCCmec type III. The frequency of MRSA strains in wild hares was low, nevertheless, the presence of MRSA in game animals is considered a public health problem and may represent a route of transmission between animals and humans.
KW - Iberian hare
KW - MecA
KW - Methicillin-resistant staphylococci
KW - MRSA
KW - Wildlife
UR - http://www.scopus.com/inward/record.url?scp=85077475160&partnerID=8YFLogxK
U2 - 10.1093/femsec/fiz204
DO - 10.1093/femsec/fiz204
M3 - Article
C2 - 31855250
AN - SCOPUS:85077475160
SN - 0168-6496
VL - 96
JO - FEMS microbiology ecology
JF - FEMS microbiology ecology
IS - 1
M1 - fiz204
ER -