@article{74845819672847658e52ea72c70e9af4,
title = "Diverse specificity of cellulosome attachment to the bacterial cell surface",
abstract = "During the course of evolution, the cellulosome, one of Nature's most intricate multi-enzyme complexes, has been continuously fine-tuned to efficiently deconstruct recalcitrant carbohydrates. To facilitate the uptake of released sugars, anaerobic bacteria use highly ordered protein-protein interactions to recruit these nanomachines to the cell surface. Dockerin modules located within a non-catalytic macromolecular scaffold, whose primary role is to assemble cellulosomal enzymatic subunits, bind cohesin modules of cell envelope proteins, thereby anchoring the cellulosome onto the bacterial cell. Here we have elucidated the unique molecular mechanisms used by anaerobic bacteria for cellulosome cellular attachment. The structure and biochemical analysis of five cohesin-dockerin complexes revealed that cell surface dockerins contain two cohesin-binding interfaces, which can present different or identical specificities. In contrast to the current static model, we propose that dockerins utilize multivalent modes of cohesin recognition to recruit cellulosomes to the cell surface, a mechanism that maximises substrate access while facilitating complex assembly.",
keywords = "COHESIN-DOCKERIN COMPLEX, DUAL BINDING MODE, CLOSTRIDIUM-THERMOCELLUM, CRYSTAL-STRUCTURE, MODULES, DOMAIN",
author = "Br{\'a}s, {Joana L. A.} and Pinheiro, {Benedita A} and Kate Cameron and Fiona Cuskin and Aldino Viegas and Shabir Najmudin and Pedro Bule and Pires, {Virginia M. R.} and Rom{\~a}o, {Maria Jo{\~a}o} and Bayer, {Edward A.} and Spencer, {Holly L} and Smith, {Steven P} and Gilbert, {Harry J.} and Alves, {Victor D} and Carvalho, {Ana Lu{\'i}sa} and Fontes, {Carlos M. G. A.}",
note = "This work was supported by the EU FP7 programme under the WallTraC project (grant No. 263916) and by projects PTDC/BIA-MIC/5947/2014, RECI/BBB-BEP/0124/2012 and EXPL/BIA-MIC/1176/2012 supported by Fundacao para a Ciencia e Tecnologia (FCT-MCTES). The Research Unit UCIBIO (Unidade de Ciencias Biomoleculares Aplicadas) is financed by national funds from FCT/MCTES EC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). We thank the European Synchrotron Radiation Facility (Grenoble, France), Soleil (Saint-Aubin, France) and Diamond Light Source (Harwell, UK) for data collection and the European Community's Seventh Framework Programme (FP7/2007-2013) under BioStruct-X (grant agreement No. 283570, proposal number: Biostruct-X_ 4399) for funding.",
year = "2016",
month = dec,
day = "7",
doi = "10.1038/srep38292",
language = "English",
volume = "6",
pages = "38292",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}