Disordered microbial communities in asthmatic airways

Markus Hilty, Conor Burke, Helder Pedro, Paul Cardenas, Andy Bush, Cara Bossley, Jane Davies, Aaron Ervine, Len Poulter, Lior Pachter, Miriam F. Moffatt, William O. C. Cookson

Research output: Contribution to journalArticle

891 Citations (Scopus)

Abstract

Background: A rich microbial environment in infancy protects against asthma [1,2] and infections precipitate asthma exacerbations [3]. We compared the airway microbiota at three levels in adult patients with asthma, the related condition of COPD, and controls. We also studied bronchial lavage from asthmatic children and controls. Principal Findings: We identified 5,054 16S rRNA bacterial sequences from 43 subjects, detecting > 70% of species present. The bronchial tree was not sterile, and contained a mean of 2,000 bacterial genomes per cm(2) surface sampled. Pathogenic Proteobacteria, particularly Haemophilus spp., were much more frequent in bronchi of adult asthmatics or patients with COPD than controls. We found similar highly significant increases in Proteobacteria in asthmatic children. Conversely, Bacteroidetes, particularly Prevotella spp., were more frequent in controls than adult or child asthmatics or COPD patients. Significance: The results show the bronchial tree to contain a characteristic microbiota, and suggest that this microbiota is disturbed in asthmatic airways.
Original languageEnglish
Pages (from-to)e8578
JournalPLoS ONE
Volume5
Issue number1
DOIs
Publication statusPublished - 5 Jan 2010

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    Hilty, M., Burke, C., Pedro, H., Cardenas, P., Bush, A., Bossley, C., Davies, J., Ervine, A., Poulter, L., Pachter, L., F. Moffatt, M., & O. C. Cookson, W. (2010). Disordered microbial communities in asthmatic airways. PLoS ONE, 5(1), e8578. https://doi.org/10.1371/journal.pone.0008578