Direct evidence of the metal-free nature of sirohydrochlorin in desulfoviridin

K. K. Lai; Isabel Moura; Ming Y. Liu; Jean LeGall; Kwok To Yue

doi:10.1016/S0005-2728(05)80114-8

Direct evidence of the metal-free nature of sirohydrochlorin in desulfoviridin

K. K. Lai, Isabel Moura, Ming Y. Liu, Jean LeGall, Kwok To Yue

DQ - Departamento de Química

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We have obtained direct evidence that the majority of the sirohydrochlorin chromophore in the dissimilatory sulfite reductase desulfoviridin from Desulfovibrio gigas, is not associated with any metal. The evidence comes from resonance Raman measurements of native and deuterated samples of desulfoviridin. The breathing mode v₄ (or v*₄) at 1336 cm^-1 in the native enzyme is downshifted to 1326 cm^-1 upon deuteration. This mode is not sensitive to deuteration if a metal is present at the center of the chromophore inside protein or in solution. The results also establish the existence of exchangeable core hydrogen(s) at the pyrrolic nitrogen(s).

Original language	English
Pages (from-to)	25-27
Number of pages	3
Journal	BBA - Bioenergetics
Volume	1060
Issue number	1
DOIs	https://doi.org/10.1016/S0005-2728(05)80114-8
Publication status	Published - 27 Sep 1991

Keywords

Raman spectroscopy
Sirohydrochlorin
Sulfite reductase

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title = "Direct evidence of the metal-free nature of sirohydrochlorin in desulfoviridin",

abstract = "We have obtained direct evidence that the majority of the sirohydrochlorin chromophore in the dissimilatory sulfite reductase desulfoviridin from Desulfovibrio gigas, is not associated with any metal. The evidence comes from resonance Raman measurements of native and deuterated samples of desulfoviridin. The breathing mode v4 (or v*4) at 1336 cm-1 in the native enzyme is downshifted to 1326 cm-1 upon deuteration. This mode is not sensitive to deuteration if a metal is present at the center of the chromophore inside protein or in solution. The results also establish the existence of exchangeable core hydrogen(s) at the pyrrolic nitrogen(s).",

keywords = "Raman spectroscopy, Sirohydrochlorin, Sulfite reductase",

author = "Lai, {K. K.} and Isabel Moura and Liu, {Ming Y.} and Jean LeGall and Yue, {Kwok To}",

note = "This work was supported by the National Institutes of Health Grant GM-38555 to K.T.¥. ",

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T1 - Direct evidence of the metal-free nature of sirohydrochlorin in desulfoviridin

AU - Lai, K. K.

AU - Moura, Isabel

AU - Liu, Ming Y.

AU - LeGall, Jean

AU - Yue, Kwok To

N1 - This work was supported by the National Institutes of Health Grant GM-38555 to K.T.¥.

PY - 1991/9/27

Y1 - 1991/9/27

N2 - We have obtained direct evidence that the majority of the sirohydrochlorin chromophore in the dissimilatory sulfite reductase desulfoviridin from Desulfovibrio gigas, is not associated with any metal. The evidence comes from resonance Raman measurements of native and deuterated samples of desulfoviridin. The breathing mode v4 (or v*4) at 1336 cm-1 in the native enzyme is downshifted to 1326 cm-1 upon deuteration. This mode is not sensitive to deuteration if a metal is present at the center of the chromophore inside protein or in solution. The results also establish the existence of exchangeable core hydrogen(s) at the pyrrolic nitrogen(s).

AB - We have obtained direct evidence that the majority of the sirohydrochlorin chromophore in the dissimilatory sulfite reductase desulfoviridin from Desulfovibrio gigas, is not associated with any metal. The evidence comes from resonance Raman measurements of native and deuterated samples of desulfoviridin. The breathing mode v4 (or v*4) at 1336 cm-1 in the native enzyme is downshifted to 1326 cm-1 upon deuteration. This mode is not sensitive to deuteration if a metal is present at the center of the chromophore inside protein or in solution. The results also establish the existence of exchangeable core hydrogen(s) at the pyrrolic nitrogen(s).

KW - Raman spectroscopy

KW - Sirohydrochlorin

KW - Sulfite reductase

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DO - 10.1016/S0005-2728(05)80114-8

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Direct evidence of the metal-free nature of sirohydrochlorin in desulfoviridin

Abstract

Keywords

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