Direct Evidence for Ferrous Ion Oxidation and Incorporation in the Absence of Oxidants by Dps from Marinobacter hydrocarbonoclasticus

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Abstract

Dps proteins (DNA-binding protein from starved cells) are hollow-sphere-shaped, dodecameric enzymes found in bacteria and archaeal species. They can oxidize ferrous iron in a controlled manner using hydrogen peroxide or molecular oxygen as co-substrate, and most of them confer physical protection through DNA binding. Oxidized iron is stored, as a mineral core, in a central cavity. Direct evidence is now provided that, furthermore, Dps proteins containing small mineral cores can oxidize and mineralize toxic ferrous ions in anaerobic conditions and in the absence of any additional aqueous oxidant co-substrate. Dps proteins containing cores of 24 irons per dodecamer can oxidize about 5 ferrous irons per dodecamer, with that number approximately doubling for protein particles containing in average 96 irons per protein. This additional activity carries importance as it can be a detoxification mechanism present during anaerobic or oxygen-limited growth conditions.

Original languageEnglish
Pages (from-to)1013-1018
Number of pages6
JournalAngewandte Chemie - International Edition
Volume58
Issue number4
DOIs
Publication statusPublished - 21 Jan 2019

Fingerprint

Oxidants
Ions
Iron
Proteins
Oxidation
Minerals
DNA
Detoxification
Molecular oxygen
Poisons
DNA-Binding Proteins
Substrates
Hydrogen Peroxide
Bacteria
Hydrogen peroxide
Oxygen
Enzymes

Keywords

  • biomineralization
  • bionanocages
  • ferroxidation
  • mini-ferritin
  • Mössbauer spectroscopy

Cite this

@article{ea260f93bd134e3cb1f6dbc12995ad1e,
title = "Direct Evidence for Ferrous Ion Oxidation and Incorporation in the Absence of Oxidants by Dps from Marinobacter hydrocarbonoclasticus",
abstract = "Dps proteins (DNA-binding protein from starved cells) are hollow-sphere-shaped, dodecameric enzymes found in bacteria and archaeal species. They can oxidize ferrous iron in a controlled manner using hydrogen peroxide or molecular oxygen as co-substrate, and most of them confer physical protection through DNA binding. Oxidized iron is stored, as a mineral core, in a central cavity. Direct evidence is now provided that, furthermore, Dps proteins containing small mineral cores can oxidize and mineralize toxic ferrous ions in anaerobic conditions and in the absence of any additional aqueous oxidant co-substrate. Dps proteins containing cores of 24 irons per dodecamer can oxidize about 5 ferrous irons per dodecamer, with that number approximately doubling for protein particles containing in average 96 irons per protein. This additional activity carries importance as it can be a detoxification mechanism present during anaerobic or oxygen-limited growth conditions.",
keywords = "biomineralization, bionanocages, ferroxidation, mini-ferritin, M{\"o}ssbauer spectroscopy",
author = "Daniela Penas and Pereira, {Alice S.} and Pedro Tavares",
note = "info:eu-repo/grantAgreement/FCT/3599-PPCDT/111485/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/64248/PT# info:eu-repo/grantAgreement/FCT/5876/147218/PT# info:eu-repo/grantAgreement/FCT/5876/147412/PT# This research was supported by Fundacao para a Ciencia e Tecnologia, Ministerio da Ciencia, Tecnologia e Ensino Superior (FCT/MCTES), grant PTDC/BIA-PRO/111485/2009 to P.T., PTDC/QUI/64248/2006 (to A.S.P.). Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO is financed by national funds from FCT/MCTES (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728), UID/FIS/00068/2013 (CEFITEC). D.P. is supported by the Radiation Biology and Biophysics Doctoral Training Programme (RABBIT), Ph.D. Fellowships (SFRH/BD/52535/2014) from FCT/MCTES.",
year = "2019",
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T1 - Direct Evidence for Ferrous Ion Oxidation and Incorporation in the Absence of Oxidants by Dps from Marinobacter hydrocarbonoclasticus

AU - Penas, Daniela

AU - Pereira, Alice S.

AU - Tavares, Pedro

N1 - info:eu-repo/grantAgreement/FCT/3599-PPCDT/111485/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/64248/PT# info:eu-repo/grantAgreement/FCT/5876/147218/PT# info:eu-repo/grantAgreement/FCT/5876/147412/PT# This research was supported by Fundacao para a Ciencia e Tecnologia, Ministerio da Ciencia, Tecnologia e Ensino Superior (FCT/MCTES), grant PTDC/BIA-PRO/111485/2009 to P.T., PTDC/QUI/64248/2006 (to A.S.P.). Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO is financed by national funds from FCT/MCTES (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728), UID/FIS/00068/2013 (CEFITEC). D.P. is supported by the Radiation Biology and Biophysics Doctoral Training Programme (RABBIT), Ph.D. Fellowships (SFRH/BD/52535/2014) from FCT/MCTES.

PY - 2019/1/21

Y1 - 2019/1/21

N2 - Dps proteins (DNA-binding protein from starved cells) are hollow-sphere-shaped, dodecameric enzymes found in bacteria and archaeal species. They can oxidize ferrous iron in a controlled manner using hydrogen peroxide or molecular oxygen as co-substrate, and most of them confer physical protection through DNA binding. Oxidized iron is stored, as a mineral core, in a central cavity. Direct evidence is now provided that, furthermore, Dps proteins containing small mineral cores can oxidize and mineralize toxic ferrous ions in anaerobic conditions and in the absence of any additional aqueous oxidant co-substrate. Dps proteins containing cores of 24 irons per dodecamer can oxidize about 5 ferrous irons per dodecamer, with that number approximately doubling for protein particles containing in average 96 irons per protein. This additional activity carries importance as it can be a detoxification mechanism present during anaerobic or oxygen-limited growth conditions.

AB - Dps proteins (DNA-binding protein from starved cells) are hollow-sphere-shaped, dodecameric enzymes found in bacteria and archaeal species. They can oxidize ferrous iron in a controlled manner using hydrogen peroxide or molecular oxygen as co-substrate, and most of them confer physical protection through DNA binding. Oxidized iron is stored, as a mineral core, in a central cavity. Direct evidence is now provided that, furthermore, Dps proteins containing small mineral cores can oxidize and mineralize toxic ferrous ions in anaerobic conditions and in the absence of any additional aqueous oxidant co-substrate. Dps proteins containing cores of 24 irons per dodecamer can oxidize about 5 ferrous irons per dodecamer, with that number approximately doubling for protein particles containing in average 96 irons per protein. This additional activity carries importance as it can be a detoxification mechanism present during anaerobic or oxygen-limited growth conditions.

KW - biomineralization

KW - bionanocages

KW - ferroxidation

KW - mini-ferritin

KW - Mössbauer spectroscopy

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