Direct electrochemical generation of catalytically competent oxyferryl species of classes i and p dye decolorizing peroxidases

Magalí F. Scocozza, Lígia O. Martins, Daniel H. Murgida

Research output: Contribution to journalArticlepeer-review

Abstract

This work introduces a novel way to obtain catalytically competent oxyferryl species for two different dye-decolorizing peroxidases (DyPs) in the absence of H2O2 or any other peroxide by simply applying a reductive electrochemical potential under aerobic conditions. UV-vis and resonance Raman spectroscopies show that this method yields long-lived compounds II and I for the DyPs from Bacillus subtilis (BsDyP; Class I) and Pseudomonas putida (PpDyP; Class P), respectively. Both electrochemically generated high valent intermediates are able to oxidize ABTS at both acidic and alkaline pH. Interestingly, the electrocatalytic efficiencies obtained at pH 7.6 are very similar to the values recorded for regular catalytic ABTS/H2O2 assays at the optimal pH of the enzymes, ca. 3.7. These findings pave the way for the design of DyP-based electrocatalytic reactors operable in an extended pH range without the need of harmful reagents such as H2O2.

Original languageEnglish
Article number12532
JournalInternational Journal of Molecular Sciences
Volume22
Issue number22
DOIs
Publication statusPublished - 1 Nov 2021

Keywords

  • Compound I
  • Compound II
  • Dye-decolorizing peroxidases
  • Raman spectroscopy
  • Spec-troelectrochemistry

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