Direct analysis of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose dry blood spot enrichments by LC-MS/MS

Margarida Coelho, Vera M Mendes, Inês Sousa Lima, Fátima Oliveira Martins, Ana B Fernandes, Maria Paula Macedo, John G Jones, Bruno Manadas

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A liquid chromatography tandem mass spectrometry (LC-MS/MS) using multiple reaction monitoring (MRM) in a triple-quadrupole scan mode was developed and comprehensively validated for the determination of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose enrichments from dried blood spots (DBS) without prior derivatization. The method is demonstrated with dried blood spots obtained from rats administered with a primed-constant infusion of [U-C-13(6)]glucose and an oral glucose load enriched with [6,6-H-2(2)]glucose. The sensitivity is sufficient for analysis of the equivalent to textless5 mu L. of blood and the overall method was accurate and precise for the determination of DBS isotopic enrichments. (C) 2016 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)242-248
Number of pages7
JournalJournal of Chromatography B
Volume1022
DOIs
Publication statusPublished - 1 Jun 2016

Keywords

  • Glucose kinetics
  • Isotope dilution measurement
  • Dried blood spot
  • Animal model

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