Direct analysis of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose dry blood spot enrichments by LC-MS/MS

Margarida Coelho; Vera M Mendes; Inês Sousa Lima; Fátima Oliveira Martins; Ana B Fernandes; Maria Paula Macedo; John G Jones; Bruno Manadas

doi:10.1016/j.jchromb.2016.04.027

Direct analysis of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose dry blood spot enrichments by LC-MS/MS

Margarida Coelho, Vera M Mendes, Inês Sousa Lima, Fátima Oliveira Martins, Ana B Fernandes, Maria Paula Macedo, John G Jones, Bruno Manadas

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

A liquid chromatography tandem mass spectrometry (LC-MS/MS) using multiple reaction monitoring (MRM) in a triple-quadrupole scan mode was developed and comprehensively validated for the determination of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose enrichments from dried blood spots (DBS) without prior derivatization. The method is demonstrated with dried blood spots obtained from rats administered with a primed-constant infusion of [U-C-13(6)]glucose and an oral glucose load enriched with [6,6-H-2(2)]glucose. The sensitivity is sufficient for analysis of the equivalent to textless5 mu L. of blood and the overall method was accurate and precise for the determination of DBS isotopic enrichments. (C) 2016 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	242-248
Number of pages	7
Journal	Journal of Chromatography B
Volume	1022
DOIs	https://doi.org/10.1016/j.jchromb.2016.04.027
Publication status	Published - 1 Jun 2016

Keywords

Glucose kinetics
Isotope dilution measurement
Dried blood spot
Animal model

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@article{fe57f378d5b14563a9eccec0b6a00303,

title = "Direct analysis of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose dry blood spot enrichments by LC-MS/MS",

abstract = "A liquid chromatography tandem mass spectrometry (LC-MS/MS) using multiple reaction monitoring (MRM) in a triple-quadrupole scan mode was developed and comprehensively validated for the determination of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose enrichments from dried blood spots (DBS) without prior derivatization. The method is demonstrated with dried blood spots obtained from rats administered with a primed-constant infusion of [U-C-13(6)]glucose and an oral glucose load enriched with [6,6-H-2(2)]glucose. The sensitivity is sufficient for analysis of the equivalent to textless5 mu L. of blood and the overall method was accurate and precise for the determination of DBS isotopic enrichments. (C) 2016 Elsevier B.V. All rights reserved.",

keywords = "Glucose kinetics, Isotope dilution measurement, Dried blood spot, Animal model",

author = "Margarida Coelho and Mendes, {Vera M} and Lima, {In{\^e}s Sousa} and Martins, {F{\'a}tima Oliveira} and Fernandes, {Ana B} and Macedo, {Maria Paula} and Jones, {John G} and Bruno Manadas",

year = "2016",

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doi = "10.1016/j.jchromb.2016.04.027",

language = "English",

volume = "1022",

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journal = "Journal of Chromatography B",

issn = "1570-0232",

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}

Direct analysis of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose dry blood spot enrichments by LC-MS/MS. / Coelho, Margarida; Mendes, Vera M; Lima, Inês Sousa ; Martins, Fátima Oliveira ; Fernandes, Ana B ; Macedo, Maria Paula; Jones, John G; Manadas, Bruno.

In: Journal of Chromatography B, Vol. 1022, 01.06.2016, p. 242-248.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Direct analysis of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose dry blood spot enrichments by LC-MS/MS

AU - Coelho, Margarida

AU - Mendes, Vera M

AU - Lima, Inês Sousa

AU - Martins, Fátima Oliveira

AU - Fernandes, Ana B

AU - Macedo, Maria Paula

AU - Jones, John G

AU - Manadas, Bruno

PY - 2016/6/1

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AB - A liquid chromatography tandem mass spectrometry (LC-MS/MS) using multiple reaction monitoring (MRM) in a triple-quadrupole scan mode was developed and comprehensively validated for the determination of [6,6-H-2(2)]glucose and [U-C-13(6)]glucose enrichments from dried blood spots (DBS) without prior derivatization. The method is demonstrated with dried blood spots obtained from rats administered with a primed-constant infusion of [U-C-13(6)]glucose and an oral glucose load enriched with [6,6-H-2(2)]glucose. The sensitivity is sufficient for analysis of the equivalent to textless5 mu L. of blood and the overall method was accurate and precise for the determination of DBS isotopic enrichments. (C) 2016 Elsevier B.V. All rights reserved.

KW - Glucose kinetics

KW - Isotope dilution measurement

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KW - Animal model

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