TY - JOUR
T1 - Differential regulation of antioxidant machinery on grass pea partial resistance against powdery mildew and rust pathogens
AU - Martins, Davide Coelho
AU - Mendes, Francisco A.
AU - Araújo, Susana de Sousa
AU - Rubiales, Diego
AU - Vaz Patto, Maria Carlota
N1 - Funding Information:
This work was supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia through the grant PD/BD/128498/2017 (D.C.M.) and grant 2022.11911.BD (F.A.M.), the R&D research Unit GREEN-IT \u2013 Bioresources for Sustainability (UID/04551/2020, UIDP/04551/2020), and the Associated Laboratory LS4Future (LA/P/0087/2020), and by the European Union through the DIVINFOOD project (Horizon 2020, grant agreement 10383). DCM acknowledges the financial support from national funds throught FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020 (DOI: 10.54499/UIDB/00690/2020), UIDP/00690/2020 (DOI: 10.54499/UIDP/00690/2020), and SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020). DR acknowledges the financial support from Spanish project PID2023-146215OB-I00 (MCIN/AEI/10.13039/501100011033).
Publisher Copyright:
© 2025 The Authors
PY - 2025/7
Y1 - 2025/7
N2 - Powdery mildew (Erysiphe pisi, E. trifolii) and rust (Uromyces pisi) are important diseases affecting grass pea (Lathyrus sativus). This study investigates grass pea's histological, enzymatic, and metabolic responses to these pathogens using accessions with contrasting resistance. Partially resistant (PR) accessions exhibited smaller fungal colonies from 48 h after inoculation (HAI) onwards. Enhanced superoxide dismutase (SOD) activity was observed as early as 12 HAI in PR accessions against both powdery mildews, associated with increased ascorbate peroxidase (APX) and catalase (CAT) activity in E. trifolii and E. pisi infections, respectively. Moreover, phenolic compounds and flavonoids accumulated in E. trifolii-infected PR accessions (6–48 HAI). For rust, APX activity rose at 48 HAI in PR accessions. These findings suggest that partial resistance (PR) in grass pea is characterized by restricted pathogen invasion and a dynamic regulation of reactive oxygen species (ROS)-scavenging enzymes, with responses varying across pathosystems. This highlights the importance of pathogen-specific selection strategies to minimize the risk of resistance breakdown and promote durable disease resistance in breeding programs.
AB - Powdery mildew (Erysiphe pisi, E. trifolii) and rust (Uromyces pisi) are important diseases affecting grass pea (Lathyrus sativus). This study investigates grass pea's histological, enzymatic, and metabolic responses to these pathogens using accessions with contrasting resistance. Partially resistant (PR) accessions exhibited smaller fungal colonies from 48 h after inoculation (HAI) onwards. Enhanced superoxide dismutase (SOD) activity was observed as early as 12 HAI in PR accessions against both powdery mildews, associated with increased ascorbate peroxidase (APX) and catalase (CAT) activity in E. trifolii and E. pisi infections, respectively. Moreover, phenolic compounds and flavonoids accumulated in E. trifolii-infected PR accessions (6–48 HAI). For rust, APX activity rose at 48 HAI in PR accessions. These findings suggest that partial resistance (PR) in grass pea is characterized by restricted pathogen invasion and a dynamic regulation of reactive oxygen species (ROS)-scavenging enzymes, with responses varying across pathosystems. This highlights the importance of pathogen-specific selection strategies to minimize the risk of resistance breakdown and promote durable disease resistance in breeding programs.
KW - Antioxidant metabolites and enzymes
KW - Biotic stress
KW - Erysiphe pisi
KW - Erysiphe trifolii
KW - Lathyrus sativus Uromyces pisi
UR - https://www.scopus.com/pages/publications/105003967351
U2 - 10.1016/j.pmpp.2025.102717
DO - 10.1016/j.pmpp.2025.102717
M3 - Article
AN - SCOPUS:105003967351
SN - 0885-5765
VL - 138
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
M1 - 102717
ER -