Diagnosis of canine leishmaniasis: Conventional and molecular techniques using different tissues.

Carla Maia; João Ramada; J.M. Cristovao; Luzia Augusta Pires Gonçalves; Lenea Maria da Graça Campino

doi:10.1016/j.tvjl.2007.08.009

Diagnosis of canine leishmaniasis: Conventional and molecular techniques using different tissues.

Carla Maia, João Ramada, J.M. Cristovao, Luzia Augusta Pires Gonçalves, Lenea Maria da Graça Campino

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Abstract

Serology, bone marrow (BM)-, lymph node (LN)- and whole blood-polymerase chain reaction (PCR) were evaluated as potential reference tests for the diagnosis of canine leishmaniasis. A high degree of agreement ( >= 91.0%) was observed between Leishmania cultures and serology or BM/LN-PCR. In the light of these results as well as the access to biological test material and the cost of each method, LN-PCR is recommended for the diagnosis or therapeutic control of canine leishmaniasis, but BM-PCR is a suitable alternative in dogs without detectable adenomegaly. For large-scale epidemiological field studies, antibody detection is appropriate and whole blood-PCR can be used to complement the serological results. (c) 2007 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	142-144
Journal	Veterinary Journal
Volume	179
Issue number	1
DOIs	https://doi.org/10.1016/j.tvjl.2007.08.009
Publication status	Published - 1 Jan 2009

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abstract = "Serology, bone marrow (BM)-, lymph node (LN)- and whole blood-polymerase chain reaction (PCR) were evaluated as potential reference tests for the diagnosis of canine leishmaniasis. A high degree of agreement ( >= 91.0%) was observed between Leishmania cultures and serology or BM/LN-PCR. In the light of these results as well as the access to biological test material and the cost of each method, LN-PCR is recommended for the diagnosis or therapeutic control of canine leishmaniasis, but BM-PCR is a suitable alternative in dogs without detectable adenomegaly. For large-scale epidemiological field studies, antibody detection is appropriate and whole blood-PCR can be used to complement the serological results. (c) 2007 Elsevier Ltd. All rights reserved.",

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AU - Maia, Carla

AU - Ramada, João

AU - Cristovao, J.M.

AU - Gonçalves, Luzia Augusta Pires

AU - Campino, Lenea Maria da Graça

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AB - Serology, bone marrow (BM)-, lymph node (LN)- and whole blood-polymerase chain reaction (PCR) were evaluated as potential reference tests for the diagnosis of canine leishmaniasis. A high degree of agreement ( >= 91.0%) was observed between Leishmania cultures and serology or BM/LN-PCR. In the light of these results as well as the access to biological test material and the cost of each method, LN-PCR is recommended for the diagnosis or therapeutic control of canine leishmaniasis, but BM-PCR is a suitable alternative in dogs without detectable adenomegaly. For large-scale epidemiological field studies, antibody detection is appropriate and whole blood-PCR can be used to complement the serological results. (c) 2007 Elsevier Ltd. All rights reserved.

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KW - Blood

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KW - Lymph

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Diagnosis of canine leishmaniasis: Conventional and molecular techniques using different tissues.

Abstract

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