Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification

Sara D. F. Santana, Vijaykumar L Dhadge, Ana Cecília Afonso Roque

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)
24 Downloads (Pure)

Abstract

Dextran-coated iron oxide magnetic particles modified with ligand 22/8, a protein A mimetic ligand, were prepared and assessed for IgG purification. Dextran was chosen as the agent to modify the surface of magnetic particles by presenting a negligible level of nonspecific adsorption. For the functionalization of the particles with the affinity ligand toward antibodies, three methods have been explored. The optimum coupling method yielded a theoretical maximum capacity for human IgG calculated as 568 ± 33 mg/g and a binding affinity constant of 7.7 × 10⁴ M⁻¹. Regeneration, recycle and reuse of particles was also highly successful for five cycles with minor loss of capacity. Moreover, this support presented specificity and effectiveness for IgG adsorption and elution at pH 11 directly from crude extracts with a final purity of 95% in the eluted fraction.
Original languageEnglish
Pages (from-to)5907-5914
JournalAcs Applied Materials & Interfaces
Volume4
Issue number11
DOIs
Publication statusPublished - 2012

Keywords

  • magnetic particles
  • dextram
  • immobilization
  • synthetic affinity ligand
  • IgG purification

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