TY - JOUR
T1 - Development of PLGA dry powder microparticles by supercritical CO2-assisted spray-drying for potential vaccine delivery to the lungs
AU - Tavares, Márcia
AU - Cabral, Renato P.
AU - Costa, Clarinda
AU - Martins, Pedro
AU - Fernandes, Alexandra R.
AU - Casimiro, Teresa
AU - Aguiar-Ricardo, Ana
N1 - sem pdf conforme despacho.
LAQV-REQUIMTE - FCT/MCTES
UID/QUI/50006/2013 ;
UCIBIO-REQUIMTE - FCT/MCTES
UID/Multi/04378/2013 ;
ERDF - POCI-01-0145-FEDER-007265, POCI-01-0145-FEDER-007728 ;
FCT-Lisbon - IF/00915/2014
PY - 2017/1/1
Y1 - 2017/1/1
N2 - In this work, biocompatible and biodegradable poly(D-L-lactide-co-glycolide) (PLGA) composite microparticles with potential use as carrier for vaccines and other drugs to the lungs were developed using supercritical CO2-assisted spray-drying (SASD). Bovine serum albumin (BSA) was chosen as model vaccine, and L-leucine as a dispersibility enhancer, and their effects on the particle characteristics were evaluated. The dry powder formulations (DPFs) were characterized in terms of their morphology and aerodynamic performance using an in vitro aerosolization study – Andersen cascade impactor (ACI) − to obtain data such as the fine particle fraction (FPF) with percentages up to 43.4%, and the mass median aerodynamic diameter (MMAD) values between the 1.7 and 3.5 μm. Additionally, pharmacokinetic and cytotoxicity studies were performed confirming that the produced particles have all the necessary requirements for potential pulmonary delivery.
AB - In this work, biocompatible and biodegradable poly(D-L-lactide-co-glycolide) (PLGA) composite microparticles with potential use as carrier for vaccines and other drugs to the lungs were developed using supercritical CO2-assisted spray-drying (SASD). Bovine serum albumin (BSA) was chosen as model vaccine, and L-leucine as a dispersibility enhancer, and their effects on the particle characteristics were evaluated. The dry powder formulations (DPFs) were characterized in terms of their morphology and aerodynamic performance using an in vitro aerosolization study – Andersen cascade impactor (ACI) − to obtain data such as the fine particle fraction (FPF) with percentages up to 43.4%, and the mass median aerodynamic diameter (MMAD) values between the 1.7 and 3.5 μm. Additionally, pharmacokinetic and cytotoxicity studies were performed confirming that the produced particles have all the necessary requirements for potential pulmonary delivery.
KW - DOE
KW - Dry powder inhalation
KW - Poly(lactic-co-glycolic acid)
KW - Pulmonary drug delivery
KW - Supercritical assisted atomization
UR - http://www.scopus.com/inward/record.url?scp=85020706929&partnerID=8YFLogxK
U2 - 10.1016/j.supflu.2017.06.004
DO - 10.1016/j.supflu.2017.06.004
M3 - Article
AN - SCOPUS:85020706929
SN - 0896-8446
VL - 128
SP - 235
EP - 243
JO - Journal of Supercritical Fluids
JF - Journal of Supercritical Fluids
ER -
