Development of an optical immunosensor based on the fluorescence of Cyanine-5 for veterinarian diagnostics.

Marta Silva, Helder Cruz, Osvaldo Rossetti, Alicia Arese, Abel Oliva

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A model optical immunosensor was developed to quantify an antibody present in a sample by measuring the fluorescence of Cyanine-5 conjugated with the antibody, using a competitive and a sandwich immunoreaction configuration, with the antigen immobilised in controlled pore glass beads. At pH 2, 94% of the antigen-antibody complex was dissociated, allowing reutilisation. Photobleaching had no effect on the fluorescence. This model system was used to detect Brucella sp. infection and could quantify anti-Brucella sp. antibodies in ovine serum samples in the range from 0.005 to 0.11 mg ml(-1).

Original languageEnglish
Pages (from-to)993-997
Number of pages5
JournalBiotechnology Letters
Volume26
Issue number12
DOIs
Publication statusPublished - 1 Jun 2004

Keywords

  • fluorescent dye
  • BP26 protein, Brucella
  • carbocyanine
  • cyanine dye 5
  • membrane protein

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