Development of an optical immunosensor based on the fluorescence of Cyanine-5 for veterinarian diagnostics.

Marta Silva; Helder Cruz; Osvaldo Rossetti; Alicia Arese; Abel Oliva

doi:10.1023/B:BILE.0000030046.96039.e8

Development of an optical immunosensor based on the fluorescence of Cyanine-5 for veterinarian diagnostics.

Marta Silva, Helder Cruz, Osvaldo Rossetti, Alicia Arese, Abel Oliva

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A model optical immunosensor was developed to quantify an antibody present in a sample by measuring the fluorescence of Cyanine-5 conjugated with the antibody, using a competitive and a sandwich immunoreaction configuration, with the antigen immobilised in controlled pore glass beads. At pH 2, 94% of the antigen-antibody complex was dissociated, allowing reutilisation. Photobleaching had no effect on the fluorescence. This model system was used to detect Brucella sp. infection and could quantify anti-Brucella sp. antibodies in ovine serum samples in the range from 0.005 to 0.11 mg ml(-1).

Original language	English
Pages (from-to)	993-997
Number of pages	5
Journal	Biotechnology Letters
Volume	26
Issue number	12
DOIs	https://doi.org/10.1023/B:BILE.0000030046.96039.e8
Publication status	Published - 1 Jun 2004

Keywords

fluorescent dye
BP26 protein, Brucella
carbocyanine
cyanine dye 5
membrane protein

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10.1023/B:BILE.0000030046.96039.e8Licence: CC BY

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title = "Development of an optical immunosensor based on the fluorescence of Cyanine-5 for veterinarian diagnostics.",

abstract = "A model optical immunosensor was developed to quantify an antibody present in a sample by measuring the fluorescence of Cyanine-5 conjugated with the antibody, using a competitive and a sandwich immunoreaction configuration, with the antigen immobilised in controlled pore glass beads. At pH 2, 94% of the antigen-antibody complex was dissociated, allowing reutilisation. Photobleaching had no effect on the fluorescence. This model system was used to detect Brucella sp. infection and could quantify anti-Brucella sp. antibodies in ovine serum samples in the range from 0.005 to 0.11 mg ml(-1).",

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AU - Silva, Marta

AU - Cruz, Helder

AU - Rossetti, Osvaldo

AU - Arese, Alicia

AU - Oliva, Abel

PY - 2004/6/1

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AB - A model optical immunosensor was developed to quantify an antibody present in a sample by measuring the fluorescence of Cyanine-5 conjugated with the antibody, using a competitive and a sandwich immunoreaction configuration, with the antigen immobilised in controlled pore glass beads. At pH 2, 94% of the antigen-antibody complex was dissociated, allowing reutilisation. Photobleaching had no effect on the fluorescence. This model system was used to detect Brucella sp. infection and could quantify anti-Brucella sp. antibodies in ovine serum samples in the range from 0.005 to 0.11 mg ml(-1).

KW - fluorescent dye

KW - BP26 protein, Brucella

KW - carbocyanine

KW - cyanine dye 5

KW - membrane protein

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Development of an optical immunosensor based on the fluorescence of Cyanine-5 for veterinarian diagnostics.

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Keywords

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