Development of a novel electrochemical biosensor based on plastic antibodies for detection of STEAP1 biomarker in cancer

Margarida Carvalho, Rui M. Gomes, Sandra Moreira Rocha, Jorge Barroca-Ferreira, Cláudio J. Maia, Lucía Guillade, Miguel A. Correa-Duarte, Luís A. Passarinha, Felismina T. C. Moreira

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Abstract

STEAP1 is a cell surface protein of the STEAP family whose main function focuses on intercellular communication and cell growth. STEAP1 is considered a promising putative biomarker and a candidate target for prostate cancer treatment. For specific and selective detection of STEAP1, a molecularly imprinted polymers (MIP) was developed on a screen-printed electrode (C-SPE) whose surface was modified with a nanocomposite based on carbon nanotubes decorated with dendritic platinum nanoparticles (CNTs- PAH /Pt). Then, the MIPs were produced on the modified C-SPE by electropolymerization of a mixture of STEAP1 and a monomer (pyrrole-2-carboxylic acid). Then, the protein was removed from the polymeric network by enzymatic treatment with trypsin, which created the specific template cavities for further STEAP1 detection. Electrochemical techniques such as EIS and CV were used to follow the chemical modification steps of C-SPE. The analytical performance of the biosensor was evaluated by SWV in PBS buffer and in lysates of neoplastic prostate cancer cells (LNCaP) extracts. The MIP material showing a linear range from 130 pg/ml to 13 µg/ml. Overall, the biosensor exhibits essential properties such as selectivity, sensitivity and reproducibility for its application in medical and clinical research diagnosis and/or prognosis of prostate cancer.

Original languageEnglish
Article number108461
Number of pages8
JournalBioelectrochemistry
Volume152
DOIs
Publication statusPublished - Aug 2023

Keywords

  • Biosensor
  • Molecularly imprinted polymers
  • Prostate cancer
  • STEAP1

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