Development of a monoclonal antibody TAS-ELISA assay for detection of Phaeomoniella chlamydospora

Fernando Cardoso, Teresa Nascimento, Helena Oliveira

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Phaeomoniella chlamydospora is an important tracheomycotic fungus involved in different syndromes of the esca disease complex affecting young and mature grapevine plants. Although grapevine planting material is frequently infected by this pathogen, no regular screening to detect the fungus is carried out, mainly due to the lack of fast, sensitive and affordable methods for large-scale application. A monoclonal antibody-based triple antibody sandwich (TAS) enzyme-linked immunosorbent assay (ELISA) was developed to specifically detect Pa. chlamydospora from grapevine wood tissues. This assay could be especially useful for large-scale application in nurseries, to ensure Pa. chlamydospora-free grapevine stocks, and thus contributing to the production of healthy propagation material.

Original languageEnglish
Pages (from-to)194-201
Number of pages8
JournalPhytopathologia Mediterranea
Volume53
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • Esca proper
  • Grapevine wood streaking
  • Leaf stripe disease
  • Petri disease
  • Vitis vinifera

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