Development of a DNA Biodosimeter for UV radiation

Telma S. Marques, Filipa Pires, Gonçalo Magalhães-Mota, Paulo A. Ribeiro, Maria Raposo, Nigel Mason

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Ultraviolet (UV) radiation has a strong influence in the damage of deoxyribonucleic acid (DNA). In this work, the possibility of a DNA UV radiation dosimeter is evaluated. For that, calf thymus DNA samples, thin films and aqueous solutions, were irradiated with 254 nm wavelength light during different periods of time, being the damage caused by the irradiation analysed by both UV-visible and infrared spectroscopies. As the DNA is a polyelectrolyte, the pH of the DNA samples was also considered as a variable. Results demonstrated that damage in DNA takes place in both thin films and solutions when irradiated at 254 nm, as revealed by a consistent decay in measured absorbance values. However, DNA solutions were seen to give more reliable as the induced damage is easily measured. For this case, the absorbance at 260 nm was seen to exponentially decrease with the irradiation time as a result of radiation damage with the kinetics damage strongly dependent of pH. Consequently, the lifetime of such dosimeter device can be chosen by changing the pH of aqueous solutions.

Original languageEnglish
Title of host publicationPHOTOPTICS 2018 - Proceedings of the 6th International Conference on Photonics, Optics and Laser Technology
EditorsPaulo A. Ribeiro, Maria Raposo
PublisherSciTePress
Pages328-333
Number of pages6
Volume2017-January
ISBN (Electronic)978-989758286-8
Publication statusPublished - 1 Jan 2017
Event6th International Conference on Photonics, Optics and Laser Technology, PHOTOPTICS 2018 - Funchal, Madeira, Portugal
Duration: 25 Jan 201827 Jan 2018

Conference

Conference6th International Conference on Photonics, Optics and Laser Technology, PHOTOPTICS 2018
CountryPortugal
CityFunchal, Madeira
Period25/01/1827/01/18

Keywords

  • Biodosimeters
  • DNA Calf Thymus
  • UV Radiation

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  • Cite this

    Marques, T. S., Pires, F., Magalhães-Mota, G., Ribeiro, P. A., Raposo, M., & Mason, N. (2017). Development of a DNA Biodosimeter for UV radiation. In P. A. Ribeiro, & M. Raposo (Eds.), PHOTOPTICS 2018 - Proceedings of the 6th International Conference on Photonics, Optics and Laser Technology (Vol. 2017-January, pp. 328-333). SciTePress.