TY - GEN
T1 - Development and implementation of a non-parametric/metabolic model in the process optimisation of PHA production by mixed microbial cultures
AU - Oehmen, Adrian Michael
AU - Lemos, Paulo Alexandre da Costa
AU - Reis, Maria D'ascensão Carvalho Fernandes Miranda
AU - Oliveira, Rui Manuel Freitas
PY - 2007/1/1
Y1 - 2007/1/1
N2 - In this work we study the optimization of a polyhydroxyalkanoates (PHA) production process by mixed cultures based on a detailed hybrid metabolic model. The metabolic network under consideration was first decomposed into its fundamental pathways using the elementary flux modes (EFM) technique. Then, a dynamical hybrid semi-parametric model was formulated, which allowed to identify the EFM kinetics from experimental data of 7 batch runs. The EFM fluxes were interpreted in terms of metabolic consistency. The final model allowed to characterize the metabolism dynamics, namely of how the relative weight of pathways evolves in time in a typical batch or fed-batch run. The present technique is a step forward for the integration of systems biology and bioprocess control.
AB - In this work we study the optimization of a polyhydroxyalkanoates (PHA) production process by mixed cultures based on a detailed hybrid metabolic model. The metabolic network under consideration was first decomposed into its fundamental pathways using the elementary flux modes (EFM) technique. Then, a dynamical hybrid semi-parametric model was formulated, which allowed to identify the EFM kinetics from experimental data of 7 batch runs. The EFM fluxes were interpreted in terms of metabolic consistency. The final model allowed to characterize the metabolism dynamics, namely of how the relative weight of pathways evolves in time in a typical batch or fed-batch run. The present technique is a step forward for the integration of systems biology and bioprocess control.
M3 - Conference contribution
SN - 9780080546315
VL - 24
T3 - Computer Aided Chemical Engineering
SP - 995
EP - 1000
BT - 17th European Symposium on Computer Aided Process Engineering
A2 - Pleşu, Valentin
A2 - Agachi, Paul Şerban
PB - Elsevier
T2 - 17th European Symposium on Computed Aided Process Engineering
Y2 - 27 May 2007 through 30 May 2007
ER -