TY - JOUR
T1 - Development and application of a novel histological multichrome technique for clam histopathology
AU - Costa, Pedro M.
AU - Costa, Maria Helena Ferrão Ribeiro da
PY - 2012/1/1
Y1 - 2012/1/1
N2 - A novel and expedient histological tetrachrome technique was developed and applied to whole-body sections of the clam Ruditapes decussatus (L. 1758). The technique involves fixation in Carnoy's fluid followed by immediate embedding in paraffin with staining with a combination of Alcian Blue, Periodic Acid-Schiff's, Haematoxylin and Picric Acid. Fixation and staining was perfect for all tissues and resolved good identification of Perkinsus sp. infection and high structural detail. Among the surveyed fixatives, Bouin-Hollande's fluid also provided good results, however, fixation is potentially longer, polysaccharide staining was less intense and fibres appeared to be better preserved by Carnoy's.
AB - A novel and expedient histological tetrachrome technique was developed and applied to whole-body sections of the clam Ruditapes decussatus (L. 1758). The technique involves fixation in Carnoy's fluid followed by immediate embedding in paraffin with staining with a combination of Alcian Blue, Periodic Acid-Schiff's, Haematoxylin and Picric Acid. Fixation and staining was perfect for all tissues and resolved good identification of Perkinsus sp. infection and high structural detail. Among the surveyed fixatives, Bouin-Hollande's fluid also provided good results, however, fixation is potentially longer, polysaccharide staining was less intense and fibres appeared to be better preserved by Carnoy's.
U2 - 10.1016/j.jip.2012.04.013
DO - 10.1016/j.jip.2012.04.013
M3 - Article
C2 - 22564259
SN - 0022-2011
VL - 110
SP - 411
EP - 414
JO - Journal Of Invertebrate Pathology
JF - Journal Of Invertebrate Pathology
IS - 3
ER -