Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems

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Abstract

For the first time, a systematic approach was conducted to determine the key factors influencing the kinetics of hydroxyalkanote (HA) extraction in biological systems. Six mixed microbial systems where polyhydroxyalkanoate (PHA) is produced were evaluated. Experiments were carried out for full-scale and lab-scale activated sludge systems using different configurations (containing floccular or granular sludge), as well as specific PHA accumulating cultures that contain high or low intracellular PHA fractions. The overall reaction was limited by the kinetics of the PHA hydrolysis in floccular cultures, whereas in granular cultures, it was limited by the cell lysis step. The monomeric composition of the polymer also had an impact on the HA extraction rate: higher acid concentration and a longer digestion time should be employed when cells accumulate monomers with more substituents, such as hydroxy-2-methylbutyrate (H2MB) and hydroxy-2-methylvalerate (H2MV). This study optimised the method for HA extraction, which impacts the assessment of the quantity and quality of PHA biopolymers.

Original languageEnglish
Pages (from-to)1626-1634
Number of pages9
JournalProcess Biochemistry
Volume48
Issue number11
DOIs
Publication statusPublished - 1 Nov 2013

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Polyhydroxyalkanoates
Monomers
Kinetics
Sewage
Biopolymers
Biological systems
Hydrolysis
Acids
Polymers
Digestion
Chemical analysis
Experiments

Keywords

  • Extraction and recovery
  • Polyhydroxy-2- methylbutyrate (PH2MB)
  • Polyhydroxy-2-methylvalerate (PH2MV)
  • Polyhydroxybutyrate (PHB)
  • Polyhydroxyvalerate (PHV)

Cite this

@article{2f22e3fc674f40e7acd0d733f897ab91,
title = "Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems",
abstract = "For the first time, a systematic approach was conducted to determine the key factors influencing the kinetics of hydroxyalkanote (HA) extraction in biological systems. Six mixed microbial systems where polyhydroxyalkanoate (PHA) is produced were evaluated. Experiments were carried out for full-scale and lab-scale activated sludge systems using different configurations (containing floccular or granular sludge), as well as specific PHA accumulating cultures that contain high or low intracellular PHA fractions. The overall reaction was limited by the kinetics of the PHA hydrolysis in floccular cultures, whereas in granular cultures, it was limited by the cell lysis step. The monomeric composition of the polymer also had an impact on the HA extraction rate: higher acid concentration and a longer digestion time should be employed when cells accumulate monomers with more substituents, such as hydroxy-2-methylbutyrate (H2MB) and hydroxy-2-methylvalerate (H2MV). This study optimised the method for HA extraction, which impacts the assessment of the quantity and quality of PHA biopolymers.",
keywords = "Extraction and recovery, Polyhydroxy-2- methylbutyrate (PH2MB), Polyhydroxy-2-methylvalerate (PH2MV), Polyhydroxybutyrate (PHB), Polyhydroxyvalerate (PHV)",
author = "Lanham, {Ana B.} and Ricardo, {Ana Rita} and Albuquerque, {Maria G. E.} and Filipa Pardelha and M{\'o}nica Carvalheira and Marta Coma and Joana Fradinho and Gilda Carvalho and Adrian Oehmen and Reis, {Maria A. M.}",
note = "The authors wish to thankfully acknowledge funding from the Fundacao para a Ciencia e Tecnologia through the PhD grants SFRH/BD/25275/2005, SFRH/BD29477/2006, SFRH/BD38763/2007, SFRH/BD42085/2007, SFRH/BD65113/2009, the Post-Doc grants SFRH/BPD/88382/2012, SFRH/BPD/70185/2010 and the research projects PTDC/EBB-EBI/103147/2008 and PEst-C/EQB/LA0006/2011. Marta Coma also wishes to thank the financial support of the Spanish Government through the research project MICINN-PT2009-0047 and the Luso-Espanhola integrated action E-94/10.",
year = "2013",
month = "11",
day = "1",
doi = "10.1016/j.procbio.2013.07.023",
language = "English",
volume = "48",
pages = "1626--1634",
journal = "Process Biochemistry",
issn = "0032-9592",
publisher = "Elsevier Science B.V., Amsterdam.",
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TY - JOUR

T1 - Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems

AU - Lanham, Ana B.

AU - Ricardo, Ana Rita

AU - Albuquerque, Maria G. E.

AU - Pardelha, Filipa

AU - Carvalheira, Mónica

AU - Coma, Marta

AU - Fradinho, Joana

AU - Carvalho, Gilda

AU - Oehmen, Adrian

AU - Reis, Maria A. M.

N1 - The authors wish to thankfully acknowledge funding from the Fundacao para a Ciencia e Tecnologia through the PhD grants SFRH/BD/25275/2005, SFRH/BD29477/2006, SFRH/BD38763/2007, SFRH/BD42085/2007, SFRH/BD65113/2009, the Post-Doc grants SFRH/BPD/88382/2012, SFRH/BPD/70185/2010 and the research projects PTDC/EBB-EBI/103147/2008 and PEst-C/EQB/LA0006/2011. Marta Coma also wishes to thank the financial support of the Spanish Government through the research project MICINN-PT2009-0047 and the Luso-Espanhola integrated action E-94/10.

PY - 2013/11/1

Y1 - 2013/11/1

N2 - For the first time, a systematic approach was conducted to determine the key factors influencing the kinetics of hydroxyalkanote (HA) extraction in biological systems. Six mixed microbial systems where polyhydroxyalkanoate (PHA) is produced were evaluated. Experiments were carried out for full-scale and lab-scale activated sludge systems using different configurations (containing floccular or granular sludge), as well as specific PHA accumulating cultures that contain high or low intracellular PHA fractions. The overall reaction was limited by the kinetics of the PHA hydrolysis in floccular cultures, whereas in granular cultures, it was limited by the cell lysis step. The monomeric composition of the polymer also had an impact on the HA extraction rate: higher acid concentration and a longer digestion time should be employed when cells accumulate monomers with more substituents, such as hydroxy-2-methylbutyrate (H2MB) and hydroxy-2-methylvalerate (H2MV). This study optimised the method for HA extraction, which impacts the assessment of the quantity and quality of PHA biopolymers.

AB - For the first time, a systematic approach was conducted to determine the key factors influencing the kinetics of hydroxyalkanote (HA) extraction in biological systems. Six mixed microbial systems where polyhydroxyalkanoate (PHA) is produced were evaluated. Experiments were carried out for full-scale and lab-scale activated sludge systems using different configurations (containing floccular or granular sludge), as well as specific PHA accumulating cultures that contain high or low intracellular PHA fractions. The overall reaction was limited by the kinetics of the PHA hydrolysis in floccular cultures, whereas in granular cultures, it was limited by the cell lysis step. The monomeric composition of the polymer also had an impact on the HA extraction rate: higher acid concentration and a longer digestion time should be employed when cells accumulate monomers with more substituents, such as hydroxy-2-methylbutyrate (H2MB) and hydroxy-2-methylvalerate (H2MV). This study optimised the method for HA extraction, which impacts the assessment of the quantity and quality of PHA biopolymers.

KW - Extraction and recovery

KW - Polyhydroxy-2- methylbutyrate (PH2MB)

KW - Polyhydroxy-2-methylvalerate (PH2MV)

KW - Polyhydroxybutyrate (PHB)

KW - Polyhydroxyvalerate (PHV)

UR - http://www.scopus.com/inward/record.url?scp=84887050587&partnerID=8YFLogxK

U2 - 10.1016/j.procbio.2013.07.023

DO - 10.1016/j.procbio.2013.07.023

M3 - Article

VL - 48

SP - 1626

EP - 1634

JO - Process Biochemistry

JF - Process Biochemistry

SN - 0032-9592

IS - 11

ER -