TY - JOUR
T1 - Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems
AU - Lanham, Ana B.
AU - Ricardo, Ana Rita
AU - Albuquerque, Maria G. E.
AU - Pardelha, Filipa
AU - Carvalheira, Mónica
AU - Coma, Marta
AU - Fradinho, Joana
AU - Carvalho, Gilda
AU - Oehmen, Adrian
AU - Reis, Maria A. M.
N1 - The authors wish to thankfully acknowledge funding from the Fundacao para a Ciencia e Tecnologia through the PhD grants SFRH/BD/25275/2005, SFRH/BD29477/2006, SFRH/BD38763/2007, SFRH/BD42085/2007, SFRH/BD65113/2009, the Post-Doc grants SFRH/BPD/88382/2012, SFRH/BPD/70185/2010 and the research projects PTDC/EBB-EBI/103147/2008 and PEst-C/EQB/LA0006/2011. Marta Coma also wishes to thank the financial support of the Spanish Government through the research project MICINN-PT2009-0047 and the Luso-Espanhola integrated action E-94/10.
PY - 2013/11/1
Y1 - 2013/11/1
N2 - For the first time, a systematic approach was conducted to determine the key factors influencing the kinetics of hydroxyalkanote (HA) extraction in biological systems. Six mixed microbial systems where polyhydroxyalkanoate (PHA) is produced were evaluated. Experiments were carried out for full-scale and lab-scale activated sludge systems using different configurations (containing floccular or granular sludge), as well as specific PHA accumulating cultures that contain high or low intracellular PHA fractions. The overall reaction was limited by the kinetics of the PHA hydrolysis in floccular cultures, whereas in granular cultures, it was limited by the cell lysis step. The monomeric composition of the polymer also had an impact on the HA extraction rate: higher acid concentration and a longer digestion time should be employed when cells accumulate monomers with more substituents, such as hydroxy-2-methylbutyrate (H2MB) and hydroxy-2-methylvalerate (H2MV). This study optimised the method for HA extraction, which impacts the assessment of the quantity and quality of PHA biopolymers.
AB - For the first time, a systematic approach was conducted to determine the key factors influencing the kinetics of hydroxyalkanote (HA) extraction in biological systems. Six mixed microbial systems where polyhydroxyalkanoate (PHA) is produced were evaluated. Experiments were carried out for full-scale and lab-scale activated sludge systems using different configurations (containing floccular or granular sludge), as well as specific PHA accumulating cultures that contain high or low intracellular PHA fractions. The overall reaction was limited by the kinetics of the PHA hydrolysis in floccular cultures, whereas in granular cultures, it was limited by the cell lysis step. The monomeric composition of the polymer also had an impact on the HA extraction rate: higher acid concentration and a longer digestion time should be employed when cells accumulate monomers with more substituents, such as hydroxy-2-methylbutyrate (H2MB) and hydroxy-2-methylvalerate (H2MV). This study optimised the method for HA extraction, which impacts the assessment of the quantity and quality of PHA biopolymers.
KW - Extraction and recovery
KW - Polyhydroxy-2- methylbutyrate (PH2MB)
KW - Polyhydroxy-2-methylvalerate (PH2MV)
KW - Polyhydroxybutyrate (PHB)
KW - Polyhydroxyvalerate (PHV)
UR - http://www.scopus.com/inward/record.url?scp=84887050587&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2013.07.023
DO - 10.1016/j.procbio.2013.07.023
M3 - Article
AN - SCOPUS:84887050587
SN - 1359-5113
VL - 48
SP - 1626
EP - 1634
JO - Process Biochemistry
JF - Process Biochemistry
IS - 11
ER -