Detection of Rickettsia conorii israelensis DNA in the blood of a cat and a dog from southern Portugal

C Maia; JM Cristovao; André Pereira; R Parreira; L Campino

doi:10.1053/j.tcam.2019.06.001

Detection of Rickettsia conorii israelensis DNA in the blood of a cat and a dog from southern Portugal

C Maia, JM Cristovao, André Pereira, R Parreira, L Campino

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Vector-borne rickettsioses represent emerging threats to public health worldwide. The aim of this work was the screening for the presence of Rickettsia spp. in the blood of dogs and cats from southern Portugal. A PCR product of the expected size was amplified from DNA extracts obtained from blood samples of 29 out of 225 (12.9%) cats and in 2 out of 375 (.5%) dogs using genus-specific primers targeting Rickettsia gltA. Rickettsia conorii israelensis was identified by phylogenetical analysis of partial ompB sequences, amplified from blood samples taken from both a cat and a dog. The obtained results reinforce the idea that domestic animals may act as sentinels for the presence of vector-borne Rickettsia spp. in a given geographical area. In addition, rickettsioses should be included in the differential diagnosis of canine and feline vector-borne diseases.

Original language	English
Pages (from-to)	12-15
Number of pages	4
Journal	Topics in companion animal medicine
Volume	36
DOIs	https://doi.org/10.1053/j.tcam.2019.06.001
Publication status	Published - Sept 2019

Keywords

PCR
R. conorii israelensis
Rickettsia spp
Cat
Dog
Portugal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1053/j.tcam.2019.06.001

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@article{745fd4ab82d145f39edadf779cdfaed4,

title = "Detection of Rickettsia conorii israelensis DNA in the blood of a cat and a dog from southern Portugal",

abstract = "Vector-borne rickettsioses represent emerging threats to public health worldwide. The aim of this work was the screening for the presence of Rickettsia spp. in the blood of dogs and cats from southern Portugal. A PCR product of the expected size was amplified from DNA extracts obtained from blood samples of 29 out of 225 (12.9%) cats and in 2 out of 375 (.5%) dogs using genus-specific primers targeting Rickettsia gltA. Rickettsia conorii israelensis was identified by phylogenetical analysis of partial ompB sequences, amplified from blood samples taken from both a cat and a dog. The obtained results reinforce the idea that domestic animals may act as sentinels for the presence of vector-borne Rickettsia spp. in a given geographical area. In addition, rickettsioses should be included in the differential diagnosis of canine and feline vector-borne diseases.",

keywords = "PCR, R. conorii israelensis, Rickettsia spp, Cat, Dog, Portugal",

author = "C Maia and JM Cristovao and Andr{\'e} Pereira and R Parreira and L Campino",

note = "Funding Information: Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (FCT) for funds to GHTM-UID/Multi/04413/2013. CM and AP have the support of the Portuguese Ministry of Education and Science (via Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia, I.P.), through an Investigator Starting Grant ( IF/01302/2015 ) and a PhD grant ( SFRH/BD/116516/2016 ), respectively. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

month = sep,

doi = "10.1053/j.tcam.2019.06.001",

language = "English",

volume = "36",

pages = "12--15",

journal = "Topics in companion animal medicine",

issn = "1946-9837",

publisher = "W.B. Saunders Ltd",

}

TY - JOUR

T1 - Detection of Rickettsia conorii israelensis DNA in the blood of a cat and a dog from southern Portugal

AU - Maia, C

AU - Cristovao, JM

AU - Pereira, André

AU - Parreira, R

AU - Campino, L

N1 - Funding Information: Fundação para a Ciência e a Tecnologia (FCT) for funds to GHTM-UID/Multi/04413/2013. CM and AP have the support of the Portuguese Ministry of Education and Science (via Fundação para a Ciência e a Tecnologia, I.P.), through an Investigator Starting Grant ( IF/01302/2015 ) and a PhD grant ( SFRH/BD/116516/2016 ), respectively. Publisher Copyright: © 2019 Elsevier Inc.

PY - 2019/9

Y1 - 2019/9

N2 - Vector-borne rickettsioses represent emerging threats to public health worldwide. The aim of this work was the screening for the presence of Rickettsia spp. in the blood of dogs and cats from southern Portugal. A PCR product of the expected size was amplified from DNA extracts obtained from blood samples of 29 out of 225 (12.9%) cats and in 2 out of 375 (.5%) dogs using genus-specific primers targeting Rickettsia gltA. Rickettsia conorii israelensis was identified by phylogenetical analysis of partial ompB sequences, amplified from blood samples taken from both a cat and a dog. The obtained results reinforce the idea that domestic animals may act as sentinels for the presence of vector-borne Rickettsia spp. in a given geographical area. In addition, rickettsioses should be included in the differential diagnosis of canine and feline vector-borne diseases.

AB - Vector-borne rickettsioses represent emerging threats to public health worldwide. The aim of this work was the screening for the presence of Rickettsia spp. in the blood of dogs and cats from southern Portugal. A PCR product of the expected size was amplified from DNA extracts obtained from blood samples of 29 out of 225 (12.9%) cats and in 2 out of 375 (.5%) dogs using genus-specific primers targeting Rickettsia gltA. Rickettsia conorii israelensis was identified by phylogenetical analysis of partial ompB sequences, amplified from blood samples taken from both a cat and a dog. The obtained results reinforce the idea that domestic animals may act as sentinels for the presence of vector-borne Rickettsia spp. in a given geographical area. In addition, rickettsioses should be included in the differential diagnosis of canine and feline vector-borne diseases.

KW - PCR

KW - R. conorii israelensis

KW - Rickettsia spp

KW - Cat

KW - Dog

KW - Portugal

UR - https://www.sciencedirect.com/science/article/pii/S1938973619300248?via%3Dihub

U2 - 10.1053/j.tcam.2019.06.001

DO - 10.1053/j.tcam.2019.06.001

M3 - Article

C2 - 31472723

SN - 1946-9837

VL - 36

SP - 12

EP - 15

JO - Topics in companion animal medicine

JF - Topics in companion animal medicine

ER -

Detection of Rickettsia conorii israelensis DNA in the blood of a cat and a dog from southern Portugal

Abstract

Keywords

UN SDGs

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